Influence of methamphetamine on genital herpes simplex virus type 2 infection in a mouse model

Frances Valencia, Marcy J. Bubar, Gregg Milligan, Kathryn Cunningham, Nigel Bourne

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Use of the stimulant methamphetamine (METH) is increasingly common, with >35 million users worldwide. There is a known association between stimulant use and an increased incidence of HIV and other sexually transmitted infections (STIs). METH is known to have immune modulatory properties. However, the impact of METH on normal immune responses and disease pathogenesis with STIs has not been fully examined. Methods: We used a well-characterized murine model to investigate the impact of METH use on genital herpes simplex virus type 2 infection. Plaque assay and quantitative real-time polymerase chain reaction were used to measure viral replication. Cytokine bead array and enzyme-linked immunosorbent assay were used to determine levels of cytokines during host innate immune response. Results: METH treatment altered behavior, onset of clinical signs, and disease progression. METH-treated mice also had a thinned vaginal epithelium and an increase in virus present in the sensory ganglia. In addition, METH produced a local dysregulation of cytokine secretion that contrasts with its minimal impact on systemic cytokine secretion. Conclusions: Results suggest that the METH alterations of the host immune response partially contribute to enhanced genital herpes disease progression. These findings will improve understanding of METH use on host immune responses and susceptibility to disease.

Original languageEnglish (US)
Pages (from-to)720-725
Number of pages6
JournalSexually Transmitted Diseases
Volume39
Issue number9
DOIs
StatePublished - Sep 2012

Fingerprint

Herpes Genitalis
Human Herpesvirus 2
Methamphetamine
Virus Diseases
Cytokines
Sexually Transmitted Diseases
Disease Progression
Sensory Ganglia
Disease Susceptibility
Immune System Diseases
Innate Immunity
Real-Time Polymerase Chain Reaction
Epithelium
Enzyme-Linked Immunosorbent Assay
HIV
Viruses

ASJC Scopus subject areas

  • Dermatology
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Influence of methamphetamine on genital herpes simplex virus type 2 infection in a mouse model. / Valencia, Frances; Bubar, Marcy J.; Milligan, Gregg; Cunningham, Kathryn; Bourne, Nigel.

In: Sexually Transmitted Diseases, Vol. 39, No. 9, 09.2012, p. 720-725.

Research output: Contribution to journalArticle

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