Influence of type 2 T cell responses on the severity of encephalitis associated with influenza virus infection

Masahide Kaji, Makiko Kobayashi, Richard B. Pollard, Fujio Suzuki

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The role of type 2 T cell responses on the severity of post-infectious encephalitis was investigated in a mouse model of influenza virus infection. When mice were infected intracerebrally with 3.0 LD50 of A/NWS33 strain of influenza virus, they all showed clinical signs of encephalitis, and 90% of them died within 10 days of the infection. However, the post-infectious encephalitis was not demonstrated in mice exposed to 0.5 LD50 of the stone virus. The mortality rates of mice infected with 0.5 LD50 of the virus were increased to levels observed in mice exposed to 3.0 LD50 of influenza virus infection, after the administration of a mixture of interleukin (IL)-4 and IL-10 (2 ng/mouse each; immediately, 1 and 2 days after the infection). In contrast, mortality rates of mice exposed to 3.0 LD50 of influenza virus were substantially decreased when these mince were treated with a mixture of monoclonal antibodies directed against IL-4 and IL-10. A predominance of type 2 T cell responses was demonstrated in splenic T cells of mice infected with 3.0 LD50 of influenza virus, although these responses were minimal in mice infected with 0.5 LD50 of the virus. After the treatment with the mixture of type 2 cytokines, an increase in the type 2 T cell responses in mice exposed to 0.5 LD50 of the virus was shown. These results indicate that type 2 T cell responses associated with the viral infection play an important role in the severity of post-infectious encephalitis induced in mice by the intracerebral infection of influenza A virus.

Original languageEnglish (US)
Pages (from-to)180-186
Number of pages7
JournalJournal of Leukocyte Biology
Volume68
Issue number2
StatePublished - 2000

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Virus Diseases
Encephalitis
Orthomyxoviridae
Lethal Dose 50
T-Lymphocytes
Viruses
Interleukin-4
Interleukin-10
Infection
Mortality
Influenza A virus
Interleukin-2
Monoclonal Antibodies
Cytokines

Keywords

  • Influenza A virus
  • Interleukin-10
  • Interteukia-4

ASJC Scopus subject areas

  • Cell Biology

Cite this

Influence of type 2 T cell responses on the severity of encephalitis associated with influenza virus infection. / Kaji, Masahide; Kobayashi, Makiko; Pollard, Richard B.; Suzuki, Fujio.

In: Journal of Leukocyte Biology, Vol. 68, No. 2, 2000, p. 180-186.

Research output: Contribution to journalArticle

Kaji, Masahide ; Kobayashi, Makiko ; Pollard, Richard B. ; Suzuki, Fujio. / Influence of type 2 T cell responses on the severity of encephalitis associated with influenza virus infection. In: Journal of Leukocyte Biology. 2000 ; Vol. 68, No. 2. pp. 180-186.
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