Influenza virus infection of human lymphocytes occurs in the immune cell cluster of the developing antiviral response

David J. Mock, Mark W. Frampton, Joan Nichols, Frank M. Domurat, Denise J. Signs, Norbert J. Roberts

Research output: Contribution to journalArticle

Abstract

Monocytes-macrophages and lymphocytes are recruited to the respiratory tract in response to influenza virus challenge and are exposed to the virus during the establishment of immune defenses. The susceptibility of human lymphocytes to infection was assessed. The presence of monocytes-macrophages was required to attain infection of both resting and proliferating lymphocytes. Lymphocyte infection occurred in the context of immune cell clusters and was blocked by the addition of anti-intercellular adhesion molecule-1 (ICAM-1) antibody to prevent cell clustering. Both peripheral blood-derived and bronchoalveolar lymphocytes were susceptible to infection. Both CD4+ and CD8+ T lymphocytes were susceptible to influenza virus infection, and the infected CD4+ and CD8+ lymphocytes served as infectious foci for other nonpermissive or even virus-permissive cells. These data show that monocytes-macrophages and both CD4+ and CD8+ lymphocytes can become infected during the course of an immune response to influenza virus challenge. The described leukocyte interactions during infection may play an important role in the development of effective anti-influenza responses.

Original languageEnglish (US)
Article number420
JournalViruses
Volume10
Issue number8
DOIs
StatePublished - Aug 10 2018

Fingerprint

Virus Diseases
Orthomyxoviridae
Antiviral Agents
Lymphocytes
Monocytes
Infection
Macrophages
Viruses
Intercellular Adhesion Molecule-1
Respiratory System
Human Influenza
Cluster Analysis
Leukocytes
T-Lymphocytes
Antibodies

Keywords

  • Alveolar lymphocytes
  • Human lymphocytes
  • Human macrophages
  • Human monocytes
  • Immune cell clusters
  • Influenza virus

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Influenza virus infection of human lymphocytes occurs in the immune cell cluster of the developing antiviral response. / Mock, David J.; Frampton, Mark W.; Nichols, Joan; Domurat, Frank M.; Signs, Denise J.; Roberts, Norbert J.

In: Viruses, Vol. 10, No. 8, 420, 10.08.2018.

Research output: Contribution to journalArticle

Mock, David J. ; Frampton, Mark W. ; Nichols, Joan ; Domurat, Frank M. ; Signs, Denise J. ; Roberts, Norbert J. / Influenza virus infection of human lymphocytes occurs in the immune cell cluster of the developing antiviral response. In: Viruses. 2018 ; Vol. 10, No. 8.
@article{6627c90459664647b1ecddcf0f94dfc8,
title = "Influenza virus infection of human lymphocytes occurs in the immune cell cluster of the developing antiviral response",
abstract = "Monocytes-macrophages and lymphocytes are recruited to the respiratory tract in response to influenza virus challenge and are exposed to the virus during the establishment of immune defenses. The susceptibility of human lymphocytes to infection was assessed. The presence of monocytes-macrophages was required to attain infection of both resting and proliferating lymphocytes. Lymphocyte infection occurred in the context of immune cell clusters and was blocked by the addition of anti-intercellular adhesion molecule-1 (ICAM-1) antibody to prevent cell clustering. Both peripheral blood-derived and bronchoalveolar lymphocytes were susceptible to infection. Both CD4+ and CD8+ T lymphocytes were susceptible to influenza virus infection, and the infected CD4+ and CD8+ lymphocytes served as infectious foci for other nonpermissive or even virus-permissive cells. These data show that monocytes-macrophages and both CD4+ and CD8+ lymphocytes can become infected during the course of an immune response to influenza virus challenge. The described leukocyte interactions during infection may play an important role in the development of effective anti-influenza responses.",
keywords = "Alveolar lymphocytes, Human lymphocytes, Human macrophages, Human monocytes, Immune cell clusters, Influenza virus",
author = "Mock, {David J.} and Frampton, {Mark W.} and Joan Nichols and Domurat, {Frank M.} and Signs, {Denise J.} and Roberts, {Norbert J.}",
year = "2018",
month = "8",
day = "10",
doi = "10.3390/v10080420",
language = "English (US)",
volume = "10",
journal = "Viruses",
issn = "1999-4915",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "8",

}

TY - JOUR

T1 - Influenza virus infection of human lymphocytes occurs in the immune cell cluster of the developing antiviral response

AU - Mock, David J.

AU - Frampton, Mark W.

AU - Nichols, Joan

AU - Domurat, Frank M.

AU - Signs, Denise J.

AU - Roberts, Norbert J.

PY - 2018/8/10

Y1 - 2018/8/10

N2 - Monocytes-macrophages and lymphocytes are recruited to the respiratory tract in response to influenza virus challenge and are exposed to the virus during the establishment of immune defenses. The susceptibility of human lymphocytes to infection was assessed. The presence of monocytes-macrophages was required to attain infection of both resting and proliferating lymphocytes. Lymphocyte infection occurred in the context of immune cell clusters and was blocked by the addition of anti-intercellular adhesion molecule-1 (ICAM-1) antibody to prevent cell clustering. Both peripheral blood-derived and bronchoalveolar lymphocytes were susceptible to infection. Both CD4+ and CD8+ T lymphocytes were susceptible to influenza virus infection, and the infected CD4+ and CD8+ lymphocytes served as infectious foci for other nonpermissive or even virus-permissive cells. These data show that monocytes-macrophages and both CD4+ and CD8+ lymphocytes can become infected during the course of an immune response to influenza virus challenge. The described leukocyte interactions during infection may play an important role in the development of effective anti-influenza responses.

AB - Monocytes-macrophages and lymphocytes are recruited to the respiratory tract in response to influenza virus challenge and are exposed to the virus during the establishment of immune defenses. The susceptibility of human lymphocytes to infection was assessed. The presence of monocytes-macrophages was required to attain infection of both resting and proliferating lymphocytes. Lymphocyte infection occurred in the context of immune cell clusters and was blocked by the addition of anti-intercellular adhesion molecule-1 (ICAM-1) antibody to prevent cell clustering. Both peripheral blood-derived and bronchoalveolar lymphocytes were susceptible to infection. Both CD4+ and CD8+ T lymphocytes were susceptible to influenza virus infection, and the infected CD4+ and CD8+ lymphocytes served as infectious foci for other nonpermissive or even virus-permissive cells. These data show that monocytes-macrophages and both CD4+ and CD8+ lymphocytes can become infected during the course of an immune response to influenza virus challenge. The described leukocyte interactions during infection may play an important role in the development of effective anti-influenza responses.

KW - Alveolar lymphocytes

KW - Human lymphocytes

KW - Human macrophages

KW - Human monocytes

KW - Immune cell clusters

KW - Influenza virus

UR - http://www.scopus.com/inward/record.url?scp=85052117164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052117164&partnerID=8YFLogxK

U2 - 10.3390/v10080420

DO - 10.3390/v10080420

M3 - Article

VL - 10

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 8

M1 - 420

ER -