Infusion of bile from methylene dianiline-treated rats into the common bile duct injures biliary epithelial cells of recipient rats

Mary F. Kanz, Aixia Wang, Gerald A. Campbell

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Methylene dianiline (4,4′-diaminodiphenylmethane, DAPM) rapidly causes cholestasis and injury to biliary epithelial cells (BEC) in the liver and common bile duct of rats. Our objective was to determine if the proximate toxicants) was present in bile. Bile from DAPM-treated or control rats was infused through the common bile duct of untreated rats via inflow and outflow cannulas for 4 h. Cholestasis, increases in serum constituents, and intrahepatic BEC injury in the livers of DAPM-treated donor rats at 4 h were comparable to previous studies (Kanz et al., Toxicol. Appl. Pharmacol. 117 (1992) 88-97) [1]. BEC injury in the common bile duct of rats receiving DAPM bile or control bile was assessed by point counting. Percent necrosis was > 28% in the common bile duct of rats receiving DAPM bile compared to < 5% in rats receiving control bile. These results indicate that bile is a major route of BEC exposure to DAPM proximate toxicant(s) and demonstrate the utility of a new method for investigating mechanisms of biliary toxicants.

Original languageEnglish (US)
Pages (from-to)165-171
Number of pages7
JournalToxicology Letters
Volume78
Issue number2
DOIs
StatePublished - Jul 1995

Keywords

  • 4,4'-Diaminodiphenylmethane
  • Biliary epithelial cell injury
  • Methylene dianiline
  • Rat bile duct

ASJC Scopus subject areas

  • Toxicology

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