Inhalation injury to tracheal epithelium in an ovine model of cotton smoke exposure

Early phase (30 minutes)

S. Abdi, M. J. Evans, R. A. Cox, H. Lubbesmeyer, David Herndon, D. L. Traber

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The purpose of this study was to evaluate lung cell injury during the acute phase of smoke inhalation injury. A group of 10 sheep were anesthetized with halothane and pancuronium followed by endotracheal intubation. In the first experiment 5 sheep were given air (sham group) and 5 were insufflated with cooled cotton smoke with a modified bee smoker. In the second part of our study (Experiment 2) the animals were insufflated with the following number of smoke breaths: 1 x 12 (n = 3); 2 x 12 (n = 4); 3 x 12 (n = 4); 4 x 12 (n = 4); and sham control (n = 1). After 30 min the animals were killed with KCl and the trachea prepared for scanning, transmission electron, and light microscopy. Our initial observation with scanning electron microscopy revealed a large amount of mucus on the surface of the epithelia. Numerous ciliated cells had been sloughed from the epithelium and were observed on the surface of the remaining ciliated cells. The sloughed cells were intact, and the cilia remained on the apical cell surface. Light and transmission electron microscopy revealed that most goblet cells were in the process of extruding mucus. The cytoplasm of goblet and basal cells appeared normal. Ciliated cells had a slightly vesiculated cytoplasm, and many were in the process of being sloughed from the epithelial surface. In these cells desmosomal attachment had been separated. The light microscope evaluation of the tracheal epithelium showed there was no dose-dependent effect between the four treatment groups. Loss of ciliated cells represents the first morphologic indicator of injury to the tracheal epithelium after exposure to smoke, regardless of the smoke dosage used. Sloughing of intact ciliated cells indicates that the smoke had an effect on how they were attached by desmosomes to the epithelium. The mechanisms by which the smoke affects the ciliated cell adhesion is yet to be determined.

Original languageEnglish (US)
Pages (from-to)1436-1439
Number of pages4
JournalAmerican Review of Respiratory Disease
Volume142
Issue number6
StatePublished - 1990

Fingerprint

Smoke
Inhalation
Sheep
Epithelium
Wounds and Injuries
Goblet Cells
Mucus
Light
Cytoplasm
Smoke Inhalation Injury
Pancuronium
Desmosomes
Scanning Transmission Electron Microscopy
Intratracheal Intubation
Cilia
Bees
Lung Injury
Halothane
Trachea
Transmission Electron Microscopy

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Abdi, S., Evans, M. J., Cox, R. A., Lubbesmeyer, H., Herndon, D., & Traber, D. L. (1990). Inhalation injury to tracheal epithelium in an ovine model of cotton smoke exposure: Early phase (30 minutes). American Review of Respiratory Disease, 142(6), 1436-1439.

Inhalation injury to tracheal epithelium in an ovine model of cotton smoke exposure : Early phase (30 minutes). / Abdi, S.; Evans, M. J.; Cox, R. A.; Lubbesmeyer, H.; Herndon, David; Traber, D. L.

In: American Review of Respiratory Disease, Vol. 142, No. 6, 1990, p. 1436-1439.

Research output: Contribution to journalArticle

Abdi, S, Evans, MJ, Cox, RA, Lubbesmeyer, H, Herndon, D & Traber, DL 1990, 'Inhalation injury to tracheal epithelium in an ovine model of cotton smoke exposure: Early phase (30 minutes)', American Review of Respiratory Disease, vol. 142, no. 6, pp. 1436-1439.
Abdi, S. ; Evans, M. J. ; Cox, R. A. ; Lubbesmeyer, H. ; Herndon, David ; Traber, D. L. / Inhalation injury to tracheal epithelium in an ovine model of cotton smoke exposure : Early phase (30 minutes). In: American Review of Respiratory Disease. 1990 ; Vol. 142, No. 6. pp. 1436-1439.
@article{9f864d159c184afe9dcaa4b05fa88cbf,
title = "Inhalation injury to tracheal epithelium in an ovine model of cotton smoke exposure: Early phase (30 minutes)",
abstract = "The purpose of this study was to evaluate lung cell injury during the acute phase of smoke inhalation injury. A group of 10 sheep were anesthetized with halothane and pancuronium followed by endotracheal intubation. In the first experiment 5 sheep were given air (sham group) and 5 were insufflated with cooled cotton smoke with a modified bee smoker. In the second part of our study (Experiment 2) the animals were insufflated with the following number of smoke breaths: 1 x 12 (n = 3); 2 x 12 (n = 4); 3 x 12 (n = 4); 4 x 12 (n = 4); and sham control (n = 1). After 30 min the animals were killed with KCl and the trachea prepared for scanning, transmission electron, and light microscopy. Our initial observation with scanning electron microscopy revealed a large amount of mucus on the surface of the epithelia. Numerous ciliated cells had been sloughed from the epithelium and were observed on the surface of the remaining ciliated cells. The sloughed cells were intact, and the cilia remained on the apical cell surface. Light and transmission electron microscopy revealed that most goblet cells were in the process of extruding mucus. The cytoplasm of goblet and basal cells appeared normal. Ciliated cells had a slightly vesiculated cytoplasm, and many were in the process of being sloughed from the epithelial surface. In these cells desmosomal attachment had been separated. The light microscope evaluation of the tracheal epithelium showed there was no dose-dependent effect between the four treatment groups. Loss of ciliated cells represents the first morphologic indicator of injury to the tracheal epithelium after exposure to smoke, regardless of the smoke dosage used. Sloughing of intact ciliated cells indicates that the smoke had an effect on how they were attached by desmosomes to the epithelium. The mechanisms by which the smoke affects the ciliated cell adhesion is yet to be determined.",
author = "S. Abdi and Evans, {M. J.} and Cox, {R. A.} and H. Lubbesmeyer and David Herndon and Traber, {D. L.}",
year = "1990",
language = "English (US)",
volume = "142",
pages = "1436--1439",
journal = "American Journal of Respiratory and Critical Care Medicine",
issn = "1073-449X",
publisher = "American Thoracic Society",
number = "6",

}

TY - JOUR

T1 - Inhalation injury to tracheal epithelium in an ovine model of cotton smoke exposure

T2 - Early phase (30 minutes)

AU - Abdi, S.

AU - Evans, M. J.

AU - Cox, R. A.

AU - Lubbesmeyer, H.

AU - Herndon, David

AU - Traber, D. L.

PY - 1990

Y1 - 1990

N2 - The purpose of this study was to evaluate lung cell injury during the acute phase of smoke inhalation injury. A group of 10 sheep were anesthetized with halothane and pancuronium followed by endotracheal intubation. In the first experiment 5 sheep were given air (sham group) and 5 were insufflated with cooled cotton smoke with a modified bee smoker. In the second part of our study (Experiment 2) the animals were insufflated with the following number of smoke breaths: 1 x 12 (n = 3); 2 x 12 (n = 4); 3 x 12 (n = 4); 4 x 12 (n = 4); and sham control (n = 1). After 30 min the animals were killed with KCl and the trachea prepared for scanning, transmission electron, and light microscopy. Our initial observation with scanning electron microscopy revealed a large amount of mucus on the surface of the epithelia. Numerous ciliated cells had been sloughed from the epithelium and were observed on the surface of the remaining ciliated cells. The sloughed cells were intact, and the cilia remained on the apical cell surface. Light and transmission electron microscopy revealed that most goblet cells were in the process of extruding mucus. The cytoplasm of goblet and basal cells appeared normal. Ciliated cells had a slightly vesiculated cytoplasm, and many were in the process of being sloughed from the epithelial surface. In these cells desmosomal attachment had been separated. The light microscope evaluation of the tracheal epithelium showed there was no dose-dependent effect between the four treatment groups. Loss of ciliated cells represents the first morphologic indicator of injury to the tracheal epithelium after exposure to smoke, regardless of the smoke dosage used. Sloughing of intact ciliated cells indicates that the smoke had an effect on how they were attached by desmosomes to the epithelium. The mechanisms by which the smoke affects the ciliated cell adhesion is yet to be determined.

AB - The purpose of this study was to evaluate lung cell injury during the acute phase of smoke inhalation injury. A group of 10 sheep were anesthetized with halothane and pancuronium followed by endotracheal intubation. In the first experiment 5 sheep were given air (sham group) and 5 were insufflated with cooled cotton smoke with a modified bee smoker. In the second part of our study (Experiment 2) the animals were insufflated with the following number of smoke breaths: 1 x 12 (n = 3); 2 x 12 (n = 4); 3 x 12 (n = 4); 4 x 12 (n = 4); and sham control (n = 1). After 30 min the animals were killed with KCl and the trachea prepared for scanning, transmission electron, and light microscopy. Our initial observation with scanning electron microscopy revealed a large amount of mucus on the surface of the epithelia. Numerous ciliated cells had been sloughed from the epithelium and were observed on the surface of the remaining ciliated cells. The sloughed cells were intact, and the cilia remained on the apical cell surface. Light and transmission electron microscopy revealed that most goblet cells were in the process of extruding mucus. The cytoplasm of goblet and basal cells appeared normal. Ciliated cells had a slightly vesiculated cytoplasm, and many were in the process of being sloughed from the epithelial surface. In these cells desmosomal attachment had been separated. The light microscope evaluation of the tracheal epithelium showed there was no dose-dependent effect between the four treatment groups. Loss of ciliated cells represents the first morphologic indicator of injury to the tracheal epithelium after exposure to smoke, regardless of the smoke dosage used. Sloughing of intact ciliated cells indicates that the smoke had an effect on how they were attached by desmosomes to the epithelium. The mechanisms by which the smoke affects the ciliated cell adhesion is yet to be determined.

UR - http://www.scopus.com/inward/record.url?scp=0025605477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025605477&partnerID=8YFLogxK

M3 - Article

VL - 142

SP - 1436

EP - 1439

JO - American Journal of Respiratory and Critical Care Medicine

JF - American Journal of Respiratory and Critical Care Medicine

SN - 1073-449X

IS - 6

ER -