Inhaled nitric oxide does not alter endotoxin-induced nitric oxide synthase activity during rat lung perfusion

M. M. Kurrek, Leticia Castillo, K. D. Bloch, S. R. Tannenbaum, W. M. Zapol

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Nitric oxide (NO) has been demonstrated to decrease its own synthesis in tissue preparations. We tested the hypothesis that endogenous NO synthesis induced by lipopolysaccharides (LPS) would be decreased by exogenous NO during isolated lung perfusion. Rats were pretreated with either saline or LPS 48 h before lung harvest. Endogenous NO synthase activity was measured as conversion of L-[14C]-arginine to L-[14C]citrulline during 90 min of perfusion. NO (100 ppm) was added to the ventilating gas during perfusion of lungs from one group of control or LPS-treated rats. A second group of control or LPS-treated rats was exposed chronically to 100 ppm NO for the 48 h before lung harvest, in addition to receiving 100 ppm NO added to the ventilating gas during lung perfusion. We conclude that conversion of L[14C]arginine to L-[14C]citrulline was minimal in control lungs and increased in response to LPS pretreatment. NO added to the ventilating gas for the 90 min of ex vivo perfusion did not alter the rate of L- [14C]citrulline production. In vivo exposure to 100 ppm NO for 48 h did not alter the induction of inducible NO synthase activity as measured during ex vivo lung perfusion. This indicates that inhaled NO does not exert negative- feedback inhibition on inducible NO synthase in the ex vivo rat lung.

Original languageEnglish (US)
Pages (from-to)1088-1092
Number of pages5
JournalJournal of Applied Physiology
Volume79
Issue number4
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Endotoxins
Nitric Oxide Synthase
Nitric Oxide
Perfusion
Lung
Lipopolysaccharides
Citrulline
Gases
Nitric Oxide Synthase Type II
Arginine
Control Groups

Keywords

  • ex vivo rat lung
  • regulation

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Inhaled nitric oxide does not alter endotoxin-induced nitric oxide synthase activity during rat lung perfusion. / Kurrek, M. M.; Castillo, Leticia; Bloch, K. D.; Tannenbaum, S. R.; Zapol, W. M.

In: Journal of Applied Physiology, Vol. 79, No. 4, 01.01.1995, p. 1088-1092.

Research output: Contribution to journalArticle

Kurrek, M. M. ; Castillo, Leticia ; Bloch, K. D. ; Tannenbaum, S. R. ; Zapol, W. M. / Inhaled nitric oxide does not alter endotoxin-induced nitric oxide synthase activity during rat lung perfusion. In: Journal of Applied Physiology. 1995 ; Vol. 79, No. 4. pp. 1088-1092.
@article{8efb74cd23e84f7f86639cbc16053303,
title = "Inhaled nitric oxide does not alter endotoxin-induced nitric oxide synthase activity during rat lung perfusion",
abstract = "Nitric oxide (NO) has been demonstrated to decrease its own synthesis in tissue preparations. We tested the hypothesis that endogenous NO synthesis induced by lipopolysaccharides (LPS) would be decreased by exogenous NO during isolated lung perfusion. Rats were pretreated with either saline or LPS 48 h before lung harvest. Endogenous NO synthase activity was measured as conversion of L-[14C]-arginine to L-[14C]citrulline during 90 min of perfusion. NO (100 ppm) was added to the ventilating gas during perfusion of lungs from one group of control or LPS-treated rats. A second group of control or LPS-treated rats was exposed chronically to 100 ppm NO for the 48 h before lung harvest, in addition to receiving 100 ppm NO added to the ventilating gas during lung perfusion. We conclude that conversion of L[14C]arginine to L-[14C]citrulline was minimal in control lungs and increased in response to LPS pretreatment. NO added to the ventilating gas for the 90 min of ex vivo perfusion did not alter the rate of L- [14C]citrulline production. In vivo exposure to 100 ppm NO for 48 h did not alter the induction of inducible NO synthase activity as measured during ex vivo lung perfusion. This indicates that inhaled NO does not exert negative- feedback inhibition on inducible NO synthase in the ex vivo rat lung.",
keywords = "ex vivo rat lung, regulation",
author = "Kurrek, {M. M.} and Leticia Castillo and Bloch, {K. D.} and Tannenbaum, {S. R.} and Zapol, {W. M.}",
year = "1995",
month = "1",
day = "1",
doi = "10.1152/jappl.1995.79.4.1088",
language = "English (US)",
volume = "79",
pages = "1088--1092",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "4",

}

TY - JOUR

T1 - Inhaled nitric oxide does not alter endotoxin-induced nitric oxide synthase activity during rat lung perfusion

AU - Kurrek, M. M.

AU - Castillo, Leticia

AU - Bloch, K. D.

AU - Tannenbaum, S. R.

AU - Zapol, W. M.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Nitric oxide (NO) has been demonstrated to decrease its own synthesis in tissue preparations. We tested the hypothesis that endogenous NO synthesis induced by lipopolysaccharides (LPS) would be decreased by exogenous NO during isolated lung perfusion. Rats were pretreated with either saline or LPS 48 h before lung harvest. Endogenous NO synthase activity was measured as conversion of L-[14C]-arginine to L-[14C]citrulline during 90 min of perfusion. NO (100 ppm) was added to the ventilating gas during perfusion of lungs from one group of control or LPS-treated rats. A second group of control or LPS-treated rats was exposed chronically to 100 ppm NO for the 48 h before lung harvest, in addition to receiving 100 ppm NO added to the ventilating gas during lung perfusion. We conclude that conversion of L[14C]arginine to L-[14C]citrulline was minimal in control lungs and increased in response to LPS pretreatment. NO added to the ventilating gas for the 90 min of ex vivo perfusion did not alter the rate of L- [14C]citrulline production. In vivo exposure to 100 ppm NO for 48 h did not alter the induction of inducible NO synthase activity as measured during ex vivo lung perfusion. This indicates that inhaled NO does not exert negative- feedback inhibition on inducible NO synthase in the ex vivo rat lung.

AB - Nitric oxide (NO) has been demonstrated to decrease its own synthesis in tissue preparations. We tested the hypothesis that endogenous NO synthesis induced by lipopolysaccharides (LPS) would be decreased by exogenous NO during isolated lung perfusion. Rats were pretreated with either saline or LPS 48 h before lung harvest. Endogenous NO synthase activity was measured as conversion of L-[14C]-arginine to L-[14C]citrulline during 90 min of perfusion. NO (100 ppm) was added to the ventilating gas during perfusion of lungs from one group of control or LPS-treated rats. A second group of control or LPS-treated rats was exposed chronically to 100 ppm NO for the 48 h before lung harvest, in addition to receiving 100 ppm NO added to the ventilating gas during lung perfusion. We conclude that conversion of L[14C]arginine to L-[14C]citrulline was minimal in control lungs and increased in response to LPS pretreatment. NO added to the ventilating gas for the 90 min of ex vivo perfusion did not alter the rate of L- [14C]citrulline production. In vivo exposure to 100 ppm NO for 48 h did not alter the induction of inducible NO synthase activity as measured during ex vivo lung perfusion. This indicates that inhaled NO does not exert negative- feedback inhibition on inducible NO synthase in the ex vivo rat lung.

KW - ex vivo rat lung

KW - regulation

UR - http://www.scopus.com/inward/record.url?scp=0028863235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028863235&partnerID=8YFLogxK

U2 - 10.1152/jappl.1995.79.4.1088

DO - 10.1152/jappl.1995.79.4.1088

M3 - Article

C2 - 8567547

AN - SCOPUS:0028863235

VL - 79

SP - 1088

EP - 1092

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 4

ER -