Inhibition by spermine of the induction of nitric oxide synthase in J774.2 macrophages

Requirement of a serum factor

Csaba Szabo, G. J. Southan, E. Wood, C. Thiemermann, J. R. Vane

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Polyamines are endogenous regulators of various cell functions. Nitric oxide (NO) is a cytostatic and cytotoxic free radical which is produced by the inducible NO synthase (iNOS) in immuno-stimulated macrophages. We tested whether spermine modulates the induction of iNOS in J774.2 macrophages. Stimulation of macrophages by bacterial lipopolysaccharide (LPS) or γ-interferon increased the accumulation of nitrite in the culture medium. Spermine (10-6-10-4M) inhibited nitrite production without causing cytotoxicity. This inhibition of NO formation by spermine was significantly reduced when it was given 6 h after LPS. Spermine did not inhibit nitrite accumulation when foetal calf serum was omitted from the tissue culture medium. Thus, spermine is an inhibitor of the induction of iNOS) and its inhibitory activity requires the presence of a serum factor.

Original languageEnglish (US)
Pages (from-to)355-356
Number of pages2
JournalBritish Journal of Pharmacology
Volume112
Issue number2
StatePublished - 1994
Externally publishedYes

Fingerprint

Spermine
Nitric Oxide Synthase
Nitrites
Macrophages
Serum
Culture Media
Lipopolysaccharides
Polyamines
Cytostatic Agents
Nitric Oxide Synthase Type II
Interferons
Free Radicals
Nitric Oxide
spermine nitric oxide complex

Keywords

  • Lipopolysaccharide
  • Nitric oxide
  • Polyamines
  • Spermine

ASJC Scopus subject areas

  • Pharmacology

Cite this

Inhibition by spermine of the induction of nitric oxide synthase in J774.2 macrophages : Requirement of a serum factor. / Szabo, Csaba; Southan, G. J.; Wood, E.; Thiemermann, C.; Vane, J. R.

In: British Journal of Pharmacology, Vol. 112, No. 2, 1994, p. 355-356.

Research output: Contribution to journalArticle

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