Inhibition by spermine of the induction of nitric oxide synthase in J774.2 macrophages: requirement of a serum factor

Csaba Szabó, Garry J. Southan, Elizabeth Wood, Christoph Thiemermann, John R. Vane

    Research output: Contribution to journalArticle

    60 Scopus citations

    Abstract

    Polyamines are endogenous regulators of various cell functions. Nitric oxide (NO) is a cytostatic and cytotoxic free radical which is produced by the inducible NO synthase (iNOS) in immuno‐stimulated macrophages. We tested whether spermine modulates the induction of iNOS in J774.2 macrophages. Stimulation of macrophages by bacterial lipopolysaccharide (LPS) or γ‐interferon increased the accumulation of nitrite in the culture medium. Spermine (10−6 − 10−4 m) inhibited nitrite production without causing cytotoxicity. This inhibition of NO formation by spermine was significantly reduced when it was given 6 h after LPS. Spermine did not inhibit nitrite accumulation when foetal calf serum was omitted from the tissue culture medium. Thus, spermine is an inhibitor of the induction of iNOS, and its inhibitory activity requires the presence of a serum factor. 1994 British Pharmacological Society

    Original languageEnglish (US)
    Pages (from-to)355-356
    Number of pages2
    JournalBritish Journal of Pharmacology
    Volume112
    Issue number2
    DOIs
    StatePublished - Jun 1994

    Keywords

    • Nitric oxide
    • lipopolysaccharide
    • polyamines
    • spermine

    ASJC Scopus subject areas

    • Pharmacology

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