Abstract
The lithogenic potential of bile depends not only on supersaturation of solutes but also on the presence of pro- and anti-nucleating factors. For example, glycine-conjugated dihydroxy bile salt dimers are potent inhibitors of calcium hydroxyapatite precipitation that function by 'poisoning' the nascent crystal. Although most inhibitors of apatite formation are anions, theoretically polycations should also be effective, and because significant concentrations of polyamines are present in bile, we have investigated the ability of these molecules to inhibit apatite formation. In vitro, each polyamine (2-10 mmol/l) was able to inhibit apatite formation, and the inhibiting power was correlated with ionic charge. Thus putrescine (2+) was the weakest inhibitor and spermine (4+) was the strongest. Mixtures of polyamines were less effective than were the individual polyamines, except at higher concentrations. Although polyamines were effective over short periods of time (270 min), over longer times (3 days) spermine was unable to prevent apatite formation. Using infrared spectroscopy, we found no evidence for interaction between phosphate ions and spermine in solution. Taken together, these results suggest that polyamines are modest inhibitors of apatite formation that likely function by retarding the dissolution of the intermediate amorphous calcium phosphate phase.
| Original language | English |
|---|---|
| Pages (from-to) | 141-145 |
| Number of pages | 5 |
| Journal | Liver |
| Volume | 13 |
| Issue number | 3 |
| State | Published - 1993 |
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ASJC Scopus subject areas
- Hepatology
Cite this
Inhibition of calcium hydroxyapatite formation by polyamines. / Crowther, R. C.; Pritchard, C. M.; Qiu, Suimin; Soloway, R. D.
In: Liver, Vol. 13, No. 3, 1993, p. 141-145.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of calcium hydroxyapatite formation by polyamines
AU - Crowther, R. C.
AU - Pritchard, C. M.
AU - Qiu, Suimin
AU - Soloway, R. D.
PY - 1993
Y1 - 1993
N2 - The lithogenic potential of bile depends not only on supersaturation of solutes but also on the presence of pro- and anti-nucleating factors. For example, glycine-conjugated dihydroxy bile salt dimers are potent inhibitors of calcium hydroxyapatite precipitation that function by 'poisoning' the nascent crystal. Although most inhibitors of apatite formation are anions, theoretically polycations should also be effective, and because significant concentrations of polyamines are present in bile, we have investigated the ability of these molecules to inhibit apatite formation. In vitro, each polyamine (2-10 mmol/l) was able to inhibit apatite formation, and the inhibiting power was correlated with ionic charge. Thus putrescine (2+) was the weakest inhibitor and spermine (4+) was the strongest. Mixtures of polyamines were less effective than were the individual polyamines, except at higher concentrations. Although polyamines were effective over short periods of time (270 min), over longer times (3 days) spermine was unable to prevent apatite formation. Using infrared spectroscopy, we found no evidence for interaction between phosphate ions and spermine in solution. Taken together, these results suggest that polyamines are modest inhibitors of apatite formation that likely function by retarding the dissolution of the intermediate amorphous calcium phosphate phase.
AB - The lithogenic potential of bile depends not only on supersaturation of solutes but also on the presence of pro- and anti-nucleating factors. For example, glycine-conjugated dihydroxy bile salt dimers are potent inhibitors of calcium hydroxyapatite precipitation that function by 'poisoning' the nascent crystal. Although most inhibitors of apatite formation are anions, theoretically polycations should also be effective, and because significant concentrations of polyamines are present in bile, we have investigated the ability of these molecules to inhibit apatite formation. In vitro, each polyamine (2-10 mmol/l) was able to inhibit apatite formation, and the inhibiting power was correlated with ionic charge. Thus putrescine (2+) was the weakest inhibitor and spermine (4+) was the strongest. Mixtures of polyamines were less effective than were the individual polyamines, except at higher concentrations. Although polyamines were effective over short periods of time (270 min), over longer times (3 days) spermine was unable to prevent apatite formation. Using infrared spectroscopy, we found no evidence for interaction between phosphate ions and spermine in solution. Taken together, these results suggest that polyamines are modest inhibitors of apatite formation that likely function by retarding the dissolution of the intermediate amorphous calcium phosphate phase.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027169345&partnerID=8YFLogxK
M3 - Article
C2 - 8393122
AN - SCOPUS:0027169345
VL - 13
SP - 141
EP - 145
JO - Liver International
JF - Liver International
SN - 1478-3223
IS - 3
ER -