Inhibition of CD18-dependent neutrophil adherence reduces organ injury after hemorrhagic shock in primates

William Mileski, R. K. Winn, N. B. Vedder, T. H. Pohlman, J. M. Harlan, C. L. Rice

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

Neutrophil adherence or aggregation may be important in the development of organ injury after hemorrhagic shock. Monoclonal antibody (MAb) 60.3 prevents both adherence and aggregation. Therefore we investigated MAb 60.3 treatment in prevention of organ injury after hemorrhagic shock in rhesus monkeys (Macaca mulatta). We performed esophagogastroscopy and placed catheters to measure cardiac output, mean arterial pressure, arterial blood gases, and urine output. Blood was removed to decrease CO to 30% of baseline for 90 minutes. Just before resuscitation, MAb 60.3 (2 mg/kg) or saline solution (control) was administered intravenously. Monitoring and fluid resuscitation continued for 24 hours, with lactated Ringer's solution given as a maintenance infusion (4 mg/kg/hr) plus additional lactated Ringer's solution to maintain CO at preshock levels. Esophagogastroscopy was repeated 24 hours after shock. There were two deaths in the control group at about 72 hours and none in the MAb 60.3 group. MAb 60.3-treated animals required less fluid (9.6 ± 8.8 ml/kg vs 263.8 ± 225.7 ml/kg), gained less weight (0.08 ± 0.11 kg vs 0.70 ± 0.37 kg), and maintained a higher hematocrit level (35.0% ± 1.0% vs 26.9% ± 4.9%). All five control animals had gastritis; MAb 60.3-treated animals had none (p < 0.05; Fisher's exact test). Inhibition of neutrophil adherence or aggregation with MAb 60.3 at the time of resuscitation reduces fluid requirements and gastric injury in monkeys after hemorrhagic shock.

Original languageEnglish (US)
Pages (from-to)206-212
Number of pages7
JournalSurgery
Volume108
Issue number2
StatePublished - 1990
Externally publishedYes

Fingerprint

Hemorrhagic Shock
Primates
Neutrophils
Monoclonal Antibodies
Wounds and Injuries
Resuscitation
Carbon Monoxide
Macaca mulatta
Gastritis
Hematocrit
Sodium Chloride
Cardiac Output
Haplorhini
Shock
Stomach
Arterial Pressure
Catheters
Gases
Maintenance
Urine

ASJC Scopus subject areas

  • Surgery

Cite this

Mileski, W., Winn, R. K., Vedder, N. B., Pohlman, T. H., Harlan, J. M., & Rice, C. L. (1990). Inhibition of CD18-dependent neutrophil adherence reduces organ injury after hemorrhagic shock in primates. Surgery, 108(2), 206-212.

Inhibition of CD18-dependent neutrophil adherence reduces organ injury after hemorrhagic shock in primates. / Mileski, William; Winn, R. K.; Vedder, N. B.; Pohlman, T. H.; Harlan, J. M.; Rice, C. L.

In: Surgery, Vol. 108, No. 2, 1990, p. 206-212.

Research output: Contribution to journalArticle

Mileski, W, Winn, RK, Vedder, NB, Pohlman, TH, Harlan, JM & Rice, CL 1990, 'Inhibition of CD18-dependent neutrophil adherence reduces organ injury after hemorrhagic shock in primates', Surgery, vol. 108, no. 2, pp. 206-212.
Mileski, William ; Winn, R. K. ; Vedder, N. B. ; Pohlman, T. H. ; Harlan, J. M. ; Rice, C. L. / Inhibition of CD18-dependent neutrophil adherence reduces organ injury after hemorrhagic shock in primates. In: Surgery. 1990 ; Vol. 108, No. 2. pp. 206-212.
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