Inhibition of cellular Cdk2 activity blocks human cytomegalovirus replication

Wade A. Bresnahan, Istvan Boldogh, Ping Chi, E. Aubrey Thompson, Thomas Albrecht

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Human cytomegalovirus is a herpesvirus that induces numerous cellular processes upon infection. Among these are activation of cyclin-dependent kinase 2, which regulates cell cycle progression in G1 and S phase. We report here that inhibition of cellular Cdk2 activity blocks HCMV replication. Inhibition of Cdk2 activity by roscovitine inhibits HCMV DNA synthesis, production of infectious progeny, and late antigen expression in infected cells in a dose-dependent manner. HCMV replication is also inhibited by the expression of a Cdk2 dominant negative mutant, whereas expression of wild- type Cdk2 has no effect on viral replication. These data indicate that activation of cellular Cdk2 is necessary for HCMV replication.

Original languageEnglish (US)
Pages (from-to)239-247
Number of pages9
JournalVirology
Volume231
Issue number2
DOIs
StatePublished - May 12 1997

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Cyclin-Dependent Kinase 2
Herpesviridae
G1 Phase
Cytomegalovirus
S Phase
Human Activities
Cell Cycle
Antigens
DNA
Infection
roscovitine

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Inhibition of cellular Cdk2 activity blocks human cytomegalovirus replication. / Bresnahan, Wade A.; Boldogh, Istvan; Chi, Ping; Thompson, E. Aubrey; Albrecht, Thomas.

In: Virology, Vol. 231, No. 2, 12.05.1997, p. 239-247.

Research output: Contribution to journalArticle

Bresnahan, WA, Boldogh, I, Chi, P, Thompson, EA & Albrecht, T 1997, 'Inhibition of cellular Cdk2 activity blocks human cytomegalovirus replication', Virology, vol. 231, no. 2, pp. 239-247. https://doi.org/10.1006/viro.1997.8489
Bresnahan, Wade A. ; Boldogh, Istvan ; Chi, Ping ; Thompson, E. Aubrey ; Albrecht, Thomas. / Inhibition of cellular Cdk2 activity blocks human cytomegalovirus replication. In: Virology. 1997 ; Vol. 231, No. 2. pp. 239-247.
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