Inhibition of cytokine-driven human immunodeficiency virus type 1 replication by protease inhibitor

Lena Al-Harthi, Kenneth A. Roebuck, Harold Kessler, Alan Landay

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Protease inhibitors block virus maturation and prevent the spread of human immunodeficiency virus (HIV)-1 in vitro. HIV-1-positive persons produce higher levels of proinflammatory cytokines that up-regulate HIV-1 replication. For the protease inhibitor to be effective in vivo, it must be able to suppress cytokine-induced HIV-1 replication. The in vitro efficacy of protease inhibitor to block tumor necrosis factor (TNF)-α, interleukin (IL)- 6, IL-1α, and IL-1β induction of HIV-1 was investigated. While 100 U/mL of the respective cytokines induced a 208- to 22-fold increase in HIV-1 p24 production, addition of protease inhibitor completely inhibited this virus induction. The kinetics indicated a sustained HIV-1 inhibition despite high levels of endogenous TNF-α induction. Dilution of protease inhibitor led to increased HIV-1 replication. These results show that while protease inhibitor can prevent cytokine induction of HIV-1 replication, a continual effective dose is required for the inhibition to be sustained.

Original languageEnglish (US)
Pages (from-to)1175-1179
Number of pages5
JournalJournal of Infectious Diseases
Issue number5
StatePublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases


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