Inhibition of gliomagenesis and attenuation of mitotic transition by MIIP

Ping Ji, S. M. Smith, Y. Wang, R. Jiang, S. W. Song, B. Li, R. Sawaya, J. M. Bruner, J. Kuang, H. Yu, G. N. Fuller, W. Zhang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The migration and invasion inhibitor protein (MIIP, also known as IIp45) was discovered as a negative regulator of cell migration and invasion in glioma. Our previous studies have shown that the MIIP protein was reduced or undetectable in some tissue samples obtained from patients with glioblastoma. The significance of MIIP in gliomagenesis is unknown. In this study, we report that MIIP has an important role in the inhibition of gliomagenesis and attenuation of mitotic transition. Increased MIIP expression levels inhibited colony formation and cell growth of glioma cell lines in vitro, whereas decreased expression by specific small interfering RNA for MIIP resulted in increased cell growth. Expression of MIIP in a glial-specific mouse model blocked glioma development and progression, thus showing that MIIP is an inhibitor of gliomagenesis. Furthermore, we show that MIIP attenuates mitotic transition and results in increased mitotic catastrophe. The biochemical mechanism of MIIP in this process is associated with its regulation of anaphase-promoting complex (APC/C) activity. MIIP interacts directly with Cdc20, and the interaction of MIIP with Cdc20 inhibits APC/C-mediated degradation of cyclin B1. Thus, MIIP attenuates mitotic transition and increases mitotic catastrophe, thereby inhibiting glioma development and progression.

Original languageEnglish (US)
Pages (from-to)3501-3508
Number of pages8
JournalOncogene
Volume29
Issue number24
DOIs
StatePublished - Jun 17 2010
Externally publishedYes

Fingerprint

Glioma
Anaphase-Promoting Complex-Cyclosome
Cyclin B1
Glioblastoma
Growth
Neuroglia
Small Interfering RNA
Cell Movement
Proteins
Cell Line

Keywords

  • APC/C
  • Centrosome
  • Gliomagenesis
  • MIIP
  • Mitotic catastrophe
  • Mitotic transition

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Ji, P., Smith, S. M., Wang, Y., Jiang, R., Song, S. W., Li, B., ... Zhang, W. (2010). Inhibition of gliomagenesis and attenuation of mitotic transition by MIIP. Oncogene, 29(24), 3501-3508. https://doi.org/10.1038/onc.2010.114

Inhibition of gliomagenesis and attenuation of mitotic transition by MIIP. / Ji, Ping; Smith, S. M.; Wang, Y.; Jiang, R.; Song, S. W.; Li, B.; Sawaya, R.; Bruner, J. M.; Kuang, J.; Yu, H.; Fuller, G. N.; Zhang, W.

In: Oncogene, Vol. 29, No. 24, 17.06.2010, p. 3501-3508.

Research output: Contribution to journalArticle

Ji, P, Smith, SM, Wang, Y, Jiang, R, Song, SW, Li, B, Sawaya, R, Bruner, JM, Kuang, J, Yu, H, Fuller, GN & Zhang, W 2010, 'Inhibition of gliomagenesis and attenuation of mitotic transition by MIIP', Oncogene, vol. 29, no. 24, pp. 3501-3508. https://doi.org/10.1038/onc.2010.114
Ji P, Smith SM, Wang Y, Jiang R, Song SW, Li B et al. Inhibition of gliomagenesis and attenuation of mitotic transition by MIIP. Oncogene. 2010 Jun 17;29(24):3501-3508. https://doi.org/10.1038/onc.2010.114
Ji, Ping ; Smith, S. M. ; Wang, Y. ; Jiang, R. ; Song, S. W. ; Li, B. ; Sawaya, R. ; Bruner, J. M. ; Kuang, J. ; Yu, H. ; Fuller, G. N. ; Zhang, W. / Inhibition of gliomagenesis and attenuation of mitotic transition by MIIP. In: Oncogene. 2010 ; Vol. 29, No. 24. pp. 3501-3508.
@article{16e096b52e6342d9b55b19724cb538f3,
title = "Inhibition of gliomagenesis and attenuation of mitotic transition by MIIP",
abstract = "The migration and invasion inhibitor protein (MIIP, also known as IIp45) was discovered as a negative regulator of cell migration and invasion in glioma. Our previous studies have shown that the MIIP protein was reduced or undetectable in some tissue samples obtained from patients with glioblastoma. The significance of MIIP in gliomagenesis is unknown. In this study, we report that MIIP has an important role in the inhibition of gliomagenesis and attenuation of mitotic transition. Increased MIIP expression levels inhibited colony formation and cell growth of glioma cell lines in vitro, whereas decreased expression by specific small interfering RNA for MIIP resulted in increased cell growth. Expression of MIIP in a glial-specific mouse model blocked glioma development and progression, thus showing that MIIP is an inhibitor of gliomagenesis. Furthermore, we show that MIIP attenuates mitotic transition and results in increased mitotic catastrophe. The biochemical mechanism of MIIP in this process is associated with its regulation of anaphase-promoting complex (APC/C) activity. MIIP interacts directly with Cdc20, and the interaction of MIIP with Cdc20 inhibits APC/C-mediated degradation of cyclin B1. Thus, MIIP attenuates mitotic transition and increases mitotic catastrophe, thereby inhibiting glioma development and progression.",
keywords = "APC/C, Centrosome, Gliomagenesis, MIIP, Mitotic catastrophe, Mitotic transition",
author = "Ping Ji and Smith, {S. M.} and Y. Wang and R. Jiang and Song, {S. W.} and B. Li and R. Sawaya and Bruner, {J. M.} and J. Kuang and H. Yu and Fuller, {G. N.} and W. Zhang",
year = "2010",
month = "6",
day = "17",
doi = "10.1038/onc.2010.114",
language = "English (US)",
volume = "29",
pages = "3501--3508",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "24",

}

TY - JOUR

T1 - Inhibition of gliomagenesis and attenuation of mitotic transition by MIIP

AU - Ji, Ping

AU - Smith, S. M.

AU - Wang, Y.

AU - Jiang, R.

AU - Song, S. W.

AU - Li, B.

AU - Sawaya, R.

AU - Bruner, J. M.

AU - Kuang, J.

AU - Yu, H.

AU - Fuller, G. N.

AU - Zhang, W.

PY - 2010/6/17

Y1 - 2010/6/17

N2 - The migration and invasion inhibitor protein (MIIP, also known as IIp45) was discovered as a negative regulator of cell migration and invasion in glioma. Our previous studies have shown that the MIIP protein was reduced or undetectable in some tissue samples obtained from patients with glioblastoma. The significance of MIIP in gliomagenesis is unknown. In this study, we report that MIIP has an important role in the inhibition of gliomagenesis and attenuation of mitotic transition. Increased MIIP expression levels inhibited colony formation and cell growth of glioma cell lines in vitro, whereas decreased expression by specific small interfering RNA for MIIP resulted in increased cell growth. Expression of MIIP in a glial-specific mouse model blocked glioma development and progression, thus showing that MIIP is an inhibitor of gliomagenesis. Furthermore, we show that MIIP attenuates mitotic transition and results in increased mitotic catastrophe. The biochemical mechanism of MIIP in this process is associated with its regulation of anaphase-promoting complex (APC/C) activity. MIIP interacts directly with Cdc20, and the interaction of MIIP with Cdc20 inhibits APC/C-mediated degradation of cyclin B1. Thus, MIIP attenuates mitotic transition and increases mitotic catastrophe, thereby inhibiting glioma development and progression.

AB - The migration and invasion inhibitor protein (MIIP, also known as IIp45) was discovered as a negative regulator of cell migration and invasion in glioma. Our previous studies have shown that the MIIP protein was reduced or undetectable in some tissue samples obtained from patients with glioblastoma. The significance of MIIP in gliomagenesis is unknown. In this study, we report that MIIP has an important role in the inhibition of gliomagenesis and attenuation of mitotic transition. Increased MIIP expression levels inhibited colony formation and cell growth of glioma cell lines in vitro, whereas decreased expression by specific small interfering RNA for MIIP resulted in increased cell growth. Expression of MIIP in a glial-specific mouse model blocked glioma development and progression, thus showing that MIIP is an inhibitor of gliomagenesis. Furthermore, we show that MIIP attenuates mitotic transition and results in increased mitotic catastrophe. The biochemical mechanism of MIIP in this process is associated with its regulation of anaphase-promoting complex (APC/C) activity. MIIP interacts directly with Cdc20, and the interaction of MIIP with Cdc20 inhibits APC/C-mediated degradation of cyclin B1. Thus, MIIP attenuates mitotic transition and increases mitotic catastrophe, thereby inhibiting glioma development and progression.

KW - APC/C

KW - Centrosome

KW - Gliomagenesis

KW - MIIP

KW - Mitotic catastrophe

KW - Mitotic transition

UR - http://www.scopus.com/inward/record.url?scp=77953792438&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953792438&partnerID=8YFLogxK

U2 - 10.1038/onc.2010.114

DO - 10.1038/onc.2010.114

M3 - Article

VL - 29

SP - 3501

EP - 3508

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 24

ER -