Inhibition of glucose-stimulated insulin release in the perfused rat pancreas by parathyroid secretory protein-I (chromogranin-A)

G. H. Greeley, J. C. Thompson, J. Ishizuka, C. W. Cooper, M. A. Levine, S. U. Gorr, D. V. Cohn

Research output: Contribution to journalArticle

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Abstract

The effect of graded doses (10-10-10-8 M) of highly purified bovine parathyroid secretory protein-I (SP-I; chromogranin-A) or synthetic porcine pancreastatin on glucose-stimulated insulin release in the perfused rat pancreas was examined. SP-I (10-9 M) inhibited the first phase of glucose-stimulated insulin release, and 10-8 M SP-I inhibited both the first and second phases of glucose-stimulated insulin release; 10-10 M SP-I was inactive. In comparison, pancreastatin at 10-10 M inhibited the first phase of insulin release, and at 10-9 and 10-8 M, pancreastatin inhibited both phases of insulin release. The inhibition by SP-I was achieved at concentrations that normally exist in the general circulation of man. These and other data suggest that circulating SP-I plays a physiological role in the regulation of insulin secretion.

Original languageEnglish (US)
Pages (from-to)1235-1238
Number of pages4
JournalEndocrinology
Volume124
Issue number3
StatePublished - 1989

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Chromogranin A
Pancreas
Insulin
Glucose
rat chromogranin B
Swine
pancreastatin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Greeley, G. H., Thompson, J. C., Ishizuka, J., Cooper, C. W., Levine, M. A., Gorr, S. U., & Cohn, D. V. (1989). Inhibition of glucose-stimulated insulin release in the perfused rat pancreas by parathyroid secretory protein-I (chromogranin-A). Endocrinology, 124(3), 1235-1238.

Inhibition of glucose-stimulated insulin release in the perfused rat pancreas by parathyroid secretory protein-I (chromogranin-A). / Greeley, G. H.; Thompson, J. C.; Ishizuka, J.; Cooper, C. W.; Levine, M. A.; Gorr, S. U.; Cohn, D. V.

In: Endocrinology, Vol. 124, No. 3, 1989, p. 1235-1238.

Research output: Contribution to journalArticle

Greeley, GH, Thompson, JC, Ishizuka, J, Cooper, CW, Levine, MA, Gorr, SU & Cohn, DV 1989, 'Inhibition of glucose-stimulated insulin release in the perfused rat pancreas by parathyroid secretory protein-I (chromogranin-A)', Endocrinology, vol. 124, no. 3, pp. 1235-1238.
Greeley GH, Thompson JC, Ishizuka J, Cooper CW, Levine MA, Gorr SU et al. Inhibition of glucose-stimulated insulin release in the perfused rat pancreas by parathyroid secretory protein-I (chromogranin-A). Endocrinology. 1989;124(3):1235-1238.
Greeley, G. H. ; Thompson, J. C. ; Ishizuka, J. ; Cooper, C. W. ; Levine, M. A. ; Gorr, S. U. ; Cohn, D. V. / Inhibition of glucose-stimulated insulin release in the perfused rat pancreas by parathyroid secretory protein-I (chromogranin-A). In: Endocrinology. 1989 ; Vol. 124, No. 3. pp. 1235-1238.
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