Inhibition of influenza A virus infection by Galectin-9

Tomoe Hattori, Tomohiro Arikawa, Yoichiro Fujioka, Junki Maruyama, Yousuke Nakayama, Yusuke Ohba, Toshiro Niki, Tadaaki Miyazaki, Mitsuomi Hirashima, Hiroshi Kida

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Galectin-9 (Gal-9) inhibited the infection of H1N1, H3N2 and H5N1 influenza A viruses in vitro and in vivo. Fifty percent effective doses (ED50) of Gal-9 were 0.1-0.5 μM depending on virus strains in the plaque reduction assay. Gal-9 but not Gal-1 bound to the virus particles of A/Puerto Rico/8/34 (H1N1) (PR/8), resulting in inhibition of virus attachment to the host cells. Lactose but not sucrose inhibited the binding of Gal-9 to the viruses. Endogenous Gal-9 expression was detected and increased with the course of infection with influenza A viruses in mice. Fifty percent of Gal-9-transgenic mice survived after the challenge with PR/8, while all of the wild-type mice died. Gal-9 treatment of mice affected diminishing influenza virus replication in the lungs, body weight loss and the expression level of inflammatory cytokines. Combined administration of Gal-9 and oseltamivir was more effective than the use of single compound in mouse model. The present results indicate that Gal-9 is a candidate compound for influenza A virus infection therapy.

Original languageEnglish (US)
Pages (from-to)5-18
Number of pages14
JournalJapanese Journal of Veterinary Research
Volume61
Issue number1-2
StatePublished - 2013
Externally publishedYes

Keywords

  • Gal-9
  • Influenza A virus
  • Inhibition of viral growth

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint Dive into the research topics of 'Inhibition of influenza A virus infection by Galectin-9'. Together they form a unique fingerprint.

  • Cite this

    Hattori, T., Arikawa, T., Fujioka, Y., Maruyama, J., Nakayama, Y., Ohba, Y., Niki, T., Miyazaki, T., Hirashima, M., & Kida, H. (2013). Inhibition of influenza A virus infection by Galectin-9. Japanese Journal of Veterinary Research, 61(1-2), 5-18.