Inhibition of leukocyte adherence and aggregation for treatment of severe cold injury in rabbits

William Mileski, J. F. Raymond, R. K. Winn, J. M. Harlan, C. L. Rice

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We tested the hypothesis that blocking neutrophil adherence and/or aggregation reduces tissue injury that results when tissue is frozen and rewarmed. The left hindlimbs of three groups of New Zealand White rabbits were immersed in a -15°C salt water bath for 30 min to freeze the foot. The foot was rewarmed in a 39°C water bath. In two groups, adherence and aggregation were blocked with monoclonal antibody (MAb) 60.3, and the third group was treated with saline. Two of the groups were treated at the time of rewarming with either saline or MAb 60.3, and the third group received MAb 60.3 at the conclusion of rewarming. Tissue edema and tissue loss were significantly less in the two groups receiving MAb 60.3 than in the control group. Rabbits treated at the time of rewarming had less edema and tissue loss than those treated at the completion of rewarming. These studies indicate that a substantial component of severe cold injury is neutrophil mediated and occurs after rewarming.

Original languageEnglish (US)
Pages (from-to)1432-1436
Number of pages5
JournalJournal of Applied Physiology
Volume74
Issue number3
StatePublished - 1993
Externally publishedYes

Fingerprint

Rewarming
Leukocytes
Rabbits
Monoclonal Antibodies
Baths
Foot
Edema
Neutrophils
Therapeutics
Water
Hindlimb
Salts
Cold Injury
Control Groups
Wounds and Injuries

Keywords

  • CD11/CD18
  • frostbite
  • integrins
  • leukocyte adhesion molecules
  • monoclonal antibody
  • neutrophils

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Inhibition of leukocyte adherence and aggregation for treatment of severe cold injury in rabbits. / Mileski, William; Raymond, J. F.; Winn, R. K.; Harlan, J. M.; Rice, C. L.

In: Journal of Applied Physiology, Vol. 74, No. 3, 1993, p. 1432-1436.

Research output: Contribution to journalArticle

Mileski, William ; Raymond, J. F. ; Winn, R. K. ; Harlan, J. M. ; Rice, C. L. / Inhibition of leukocyte adherence and aggregation for treatment of severe cold injury in rabbits. In: Journal of Applied Physiology. 1993 ; Vol. 74, No. 3. pp. 1432-1436.
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