Inhibition of Leukocyte Adherence and Susceptibility to Infection

William Mileski, P. Sikes, L. Atiles, E. Lightfoot, P. Lipsky, C. Baxter

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Leukocyte (WBC) adherence to endothelial cells (EC) has been implicated in the pathogenesis of microvascular injury. WBC-EC adherence is largely dependent on interaction between the WBC-CD18 complex and the endothelial ligand, intercellular adhesion molecule-1 (ICAM-1). Administration of monoclonal antibodies directed against CD18 and/or ICAM-1 inhibit WBC-EC adherence and have been reported to modulate ischemia-reperfusion and inflammatory injury. We asked the question, does inhibition of WBC-EC adherence by administration of monoclonal antibody directed against either CD18 (R15.7) or against ICAM-1 (R6.5) increase susceptibility to infection. New Zealand white rabbits were shaved and injected subcutaneously on their dorsum with Staphylococcus aureus (ATCC No. 25923) at two sites each with 109, 108, 107, and 106 colony-forming units (CFUs). A second set of rabbits were injected subcutaneously with Pseudomonas aeruginosa (ATCC No. 27853) at two sites each of 108 and 107 CFUs. Animals were monitored for 1 week with daily determination of weight, temperature, WBC counts, hematocrit, and gross evidence of abscess formation. There were three blinded experimental groups; animals given R15.7 (2.0 mg/kg), animals given R6.5 (2.0 mg/kg), and controls given saline (2.0 ml/kg). Administration of the anti-CD18 antibody, R15.7, resulted in significantly increased rates of abscess formation following innoculation with S. aureus and with P. aeruginosa, compared to controls and to the animals given the antibody to ICAM-1, R6.5. The administration of R6.5 did not increase the incidence or severity of abscess formation.

Original languageEnglish (US)
Pages (from-to)349-354
Number of pages6
JournalJournal of Surgical Research
Volume54
Issue number4
DOIs
StatePublished - Apr 1993
Externally publishedYes

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Intercellular Adhesion Molecule-1
Leukocytes
Endothelial Cells
Abscess
Infection
Pseudomonas aeruginosa
Staphylococcus aureus
Stem Cells
Monoclonal Antibodies
Rabbits
Reperfusion Injury
Hematocrit
Anti-Idiotypic Antibodies
Ligands
Weights and Measures
Temperature
Antibodies
Incidence
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

Cite this

Inhibition of Leukocyte Adherence and Susceptibility to Infection. / Mileski, William; Sikes, P.; Atiles, L.; Lightfoot, E.; Lipsky, P.; Baxter, C.

In: Journal of Surgical Research, Vol. 54, No. 4, 04.1993, p. 349-354.

Research output: Contribution to journalArticle

Mileski, W, Sikes, P, Atiles, L, Lightfoot, E, Lipsky, P & Baxter, C 1993, 'Inhibition of Leukocyte Adherence and Susceptibility to Infection', Journal of Surgical Research, vol. 54, no. 4, pp. 349-354. https://doi.org/10.1006/jsre.1993.1056
Mileski, William ; Sikes, P. ; Atiles, L. ; Lightfoot, E. ; Lipsky, P. ; Baxter, C. / Inhibition of Leukocyte Adherence and Susceptibility to Infection. In: Journal of Surgical Research. 1993 ; Vol. 54, No. 4. pp. 349-354.
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