Inhibition of leukocyte adherence in a rabbit model of major thermal injury

William Mileski, B. Gates, A. Sigman, P. Sikes, L. Atiles, E. Lightfoot, P. Lipsky, C. Baxter

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Leukocytes and the process of leukocyte adherence have been implicated in the pathogenesis of organ dysfunction after ischemic injury and inflammation. We asked the question: Will inhibition of leukocyte adherence by administration of a monoclonal antibody to intercellular adhesion molecule alter the systemic response to major thermal injury? New Zealand white rabbits instrumented to measure mean arterial pressure, cardiac output, urine output, and arterial oxygenation were deeply anesthetized, and 30% total body surface area full-thickness burn was created by applying brass probes heated to 100° C to the animals' backs for 15 seconds. The animals were continuously monitored, resuscitated, and given analgesic for 24 hours. There were three experimental groups: I-controls (n = 7), anesthetized and monitored; II-30% burn (n = 7) given 30% total body surface area + vehicle (saline solution 1.0 ml/kg every 8 hours); III-30% burn + R6.5 (n = 6) animals given a monoclonal antibody (R6.5, 2.0 mg/kg every 8 hours) directed against the intercellular adhesion molecule beginning 30 minutes after burn. This model of a 30% total body surface area burn injury resulted in hypotension and hypoxemia in the burn group. The animals given the antibody R6.5 maintained higher mean arterial pressure and arterial oxygenation at several points. These results suggest that leukocytes and leukocyte adherence may be involved in the pathogenesis of the systemic sequellae of major thermal injury.

Original languageEnglish (US)
Pages (from-to)610-616
Number of pages7
JournalJournal of Burn Care and Rehabilitation
Volume14
Issue number6
DOIs
StatePublished - 1993
Externally publishedYes

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Leukocytes
Hot Temperature
Rabbits
Body Surface Area
Wounds and Injuries
Cell Adhesion Molecules
Arterial Pressure
Monoclonal Antibodies
Sodium Chloride
Cardiac Output
Hypotension
Analgesics
Urine
Inflammation
Control Groups
Antibodies

ASJC Scopus subject areas

  • Rehabilitation
  • Surgery
  • Nursing(all)
  • Health Professions(all)
  • Emergency Medicine

Cite this

Inhibition of leukocyte adherence in a rabbit model of major thermal injury. / Mileski, William; Gates, B.; Sigman, A.; Sikes, P.; Atiles, L.; Lightfoot, E.; Lipsky, P.; Baxter, C.

In: Journal of Burn Care and Rehabilitation, Vol. 14, No. 6, 1993, p. 610-616.

Research output: Contribution to journalArticle

Mileski, W, Gates, B, Sigman, A, Sikes, P, Atiles, L, Lightfoot, E, Lipsky, P & Baxter, C 1993, 'Inhibition of leukocyte adherence in a rabbit model of major thermal injury', Journal of Burn Care and Rehabilitation, vol. 14, no. 6, pp. 610-616. https://doi.org/10.1097/00004630-199311000-00005
Mileski, William ; Gates, B. ; Sigman, A. ; Sikes, P. ; Atiles, L. ; Lightfoot, E. ; Lipsky, P. ; Baxter, C. / Inhibition of leukocyte adherence in a rabbit model of major thermal injury. In: Journal of Burn Care and Rehabilitation. 1993 ; Vol. 14, No. 6. pp. 610-616.
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