Inhibition of microRNA 128 promotes excitability of cultured cortical neuronal networks

K. Melodi McSweeney, Ayal B. Gussow, Shelton S. Bradrick, Sarah A. Dugger, Sahar Gelfman, Quanli Wang, Slavé Petrovski, Wayne N. Frankel, Michael J. Boland, David B. Goldstein

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Cultured neuronal networks monitored with microelectrode arrays (MEAs) have been used widely to evaluate pharmaceutical compounds for potential neurotoxic effects. A newer application of MEAs has been in the development of in vitro models of neurological disease. Here, we directly evaluated the utility of MEAs to recapitulate in vivo phenotypes of mature microRNA-128 (miR-128) deficiency, which causes fatal seizures in mice. We show that inhibition of miR-128 results in significantly increased neuronal activity in cultured neuronal networks derived from primary mouse cortical neurons. These results support the utility of MEAs in developing in vitro models of neuroexcitability disorders, such as epilepsy, and further suggest that MEAs provide an effective tool for the rapid identification of microRNAs that promote seizures when dysregulated.

Original languageEnglish (US)
Pages (from-to)1411-1416
Number of pages6
JournalGenome Research
Volume26
Issue number10
DOIs
StatePublished - Oct 2016

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    McSweeney, K. M., Gussow, A. B., Bradrick, S. S., Dugger, S. A., Gelfman, S., Wang, Q., Petrovski, S., Frankel, W. N., Boland, M. J., & Goldstein, D. B. (2016). Inhibition of microRNA 128 promotes excitability of cultured cortical neuronal networks. Genome Research, 26(10), 1411-1416. https://doi.org/10.1101/gr.199828.115