Inhibition of polyamine synthesis impairs the secretion of atrial natriuretic peptide

Ulka R. Tipnis, Paul J. Boor

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of the present study was to evaluate in vivo the role of polyamines in the secretion of atrial natriuretic peptide (ANP). α-Difluoromethylornithine (DFMO) which inhibits ornithine decarboxylase activity and polyamine synthesis was given in drinking water and through intraperitoneal administration to Sprague-Dawley rats. Carotid artery was cannulated for collection of blood samples and measurement of blood pressure following the administration of arginine-vasopressin (AVP). Analysis of polyamines in cardiac tissue indicated that DFMO treatment decreased contents of putrescine and spermidine in cardiac tissue by 80% and 48%, respectively. Quantitation of ANP in plasma by radioimmunoassay indicated that both basal and stimulated levels of ANP in DFMO-treated animals were 21.5% and 50% of those in control rats. The administration of putrescine restored the levels of basal and AVP-stimulated levels of ANP in plasma which confirmed that DFMO effect on ANP secretion occurred specifically through the polyamine pathway.

Original languageEnglish (US)
Pages (from-to)227-236
Number of pages10
JournalRegulatory Peptides
Volume37
Issue number3
DOIs
StatePublished - Feb 18 1992

Fingerprint

Polyamines
Eflornithine
Atrial Natriuretic Factor
Putrescine
Arginine Vasopressin
Rat control
Tissue
Plasmas
Ornithine Decarboxylase
Spermidine
Blood pressure
Carotid Arteries
Drinking Water
Radioimmunoassay
Sprague Dawley Rats
Rats
Animals
Blood
Blood Pressure

Keywords

  • Atrial natriuretic peptide
  • DFMO
  • Polyamine

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Inhibition of polyamine synthesis impairs the secretion of atrial natriuretic peptide. / Tipnis, Ulka R.; Boor, Paul J.

In: Regulatory Peptides, Vol. 37, No. 3, 18.02.1992, p. 227-236.

Research output: Contribution to journalArticle

Tipnis, Ulka R. ; Boor, Paul J. / Inhibition of polyamine synthesis impairs the secretion of atrial natriuretic peptide. In: Regulatory Peptides. 1992 ; Vol. 37, No. 3. pp. 227-236.
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