Inhibition of protein-protein interaction of HER2-EGFR and HER2-HER3 by a rationally designed peptidomimetic

Sashikanth Banappagari, Miriam Corti, Seth Pincus, Seetharama Satyanarayanajois

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Protein-protein interactions (PPI) play a crucial role in many biological processes and modulation of PPI using small molecules to target hot spots has therapeutic value. As a model system we will use PPI of human epidermal growth factor receptors (EGFRs). Among the four EGFRs, EGFR-HER2 and HER2-HER3 are well known in cancer. We have designed a small molecule that is targeted to modulate HER2-mediated signaling. Our approach is novel because the small molecule designed disrupts dimerization not only of EGFR-HER2, but also of HER2-HER3. In the present study we have shown, using surface plasmon resonance analysis, that a peptidomimetic, compound 5, binds specifically to HER2 protein extracellular domain and disrupts the dimerization of EGFRs. To evaluate the effect of compound 5 on HER2 signaling in vitro, Western blot and PathHunter assays were used. Results indicated that compound 5 inhibits the phosphorylation of HER2 kinase domain and inhibits the heterodimerization in a dose-dependent manner. Molecular modeling methods were used to model the PPI of HER2-HER3 heterodimer.

Original languageEnglish (US)
Pages (from-to)594-606
Number of pages13
JournalJournal of Biomolecular Structure and Dynamics
Volume30
Issue number5
DOIs
StatePublished - 2012

Keywords

  • EGFR
  • HER2
  • Homology modeling
  • Pathhunter assay
  • Peptidomimetic
  • Protein-protein interactions
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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