Inhibition of pulmonary metastasis by Z-100, an immunomodulatory lipid-arabinomannan extracted from Mycobacterium tuberculosis, in mice inoculated with B16 melanoma

M. Kobayashi, R. B. Pollard, F. Suzuki

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The anti-metastatic effect of z-100, an immunomodulatory arabinomannan extracted from Mycobacterium tuberculosis, was investigated in mice bearing B16 melanoma cells. Treatment of BF10 mice implanted with high metastatic B16F10 melanoma cells with a 10 mg/kg dose of z-100 resulted in the reduction of experimental pulmonary metastasis as compared with that of BF10 mice treated with saline. The number of pulmonary metastatic colonies in BF1 mice (mice implanted with low metastatic B16F1 melanoma cells) was greatly increased after the inoculation of CD4+ CD11b+ CD28+ TCRαβ+ type 2 T cells (F10-T(h)2 cells) derived from BF10 mice, while only a few metastatic colonies were demonstrated in lungs of BF1 mice inoculated with naive CD4+ T cells. However, the numbers of metastatic colonies in BF1 mice were not increased when they were inoculated with the F10-T(h)2 cell fraction derived from Z-100-treated BF10 mice and the generation of F10-T(h)2 cells in BF10 mice was effectively suppressed by the z-100 treatment. These results suggest that z-100 inhibits pulmonary metastasis of B16 melanoma through the regulation of tumor-associated T(h)2 cells, which are a key cell in the acceleration of tumor metastasis.

Original languageEnglish (US)
Pages (from-to)156-163
Number of pages8
JournalAnti-Cancer Drugs
Volume8
Issue number2
DOIs
StatePublished - Jan 1 1997

Keywords

  • Arabinomannan
  • B16 melanoma
  • Immunotherapy
  • Pulmonary metastasis
  • T(h)2 cells

ASJC Scopus subject areas

  • Oncology
  • Pharmacology
  • Pharmacology (medical)
  • Cancer Research

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