Inhibition of smooth muscle cell growth in vitro by an antisense oligodeoxynucleotide released from poly(DL-lactic-co-glycolic acid) microparticles

Robert L. Cleek, Ajay A. Rege, Larry Denner, Suzanne G. Eskin, Antonios G. Mikos

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

We fabricated poly(DL-lactic-co-glycolic acid) (PLGA) 50:50 microparticles loaded with an antisense (AS) oligodeoxynucleotide (ODN) against the rat tenascin mRNA and determined the effect in vitro of the AS- ODN released on smooth muscle cell (SMC) proliferation and migration. AS-ODN was entrapped using a double-emulsion-solvent-extraction technique with high efficiency. Release of AS-ODN was characterized by a small initial-burst effect followed by a period of controlled AS-ODN release for up to 20 days. SMC proliferation studies exhibited dose-dependent growth inhibition with AS- ODN-loaded microparticles. Microparticles loaded with scrambled (SC) ODN showed less growth inhibition than AS-ODN. Moreover, only the AS-ODN-loaded microparticles inhibited migration. These results demonstrate the feasibility of entrapping an AS-ODN to rat tenascin in PLGA microparticles for controlled delivery to inhibit SMC proliferation and migration.

Original languageEnglish (US)
Pages (from-to)525-530
Number of pages6
JournalJournal of Biomedical Materials Research
Volume35
Issue number4
DOIs
StatePublished - Jun 15 1997
Externally publishedYes

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ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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