Inhibition of Upf2-Dependent Nonsense-Mediated Decay Leads to Behavioral and Neurophysiological Abnormalities by Activating the Immune Response

Jennifer L. Johnson, Loredana Stoica, Yuwei Liu, Ping Jun Zhu, Abhisek Bhattacharya, Shelly A. Buffington, Redwan Huq, N. Tony Eissa, Ola Larsson, Bo T. Porse, Deepti Domingo, Urwah Nawaz, Renee Carroll, Lachlan Jolly, Tom S. Scerri, Hyung Goo Kim, Amanda Brignell, Matthew J. Coleman, Ruth Braden, Usha KiniVictoria Jackson, Anne Baxter, Melanie Bahlo, Ingrid E. Scheffer, David J. Amor, Michael S. Hildebrand, Penelope E. Bonnen, Christine Beeton, Jozef Gecz, Angela T. Morgan, Mauro Costa-Mattioli

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In humans, disruption of nonsense-mediated decay (NMD) has been associated with neurodevelopmental disorders (NDDs) such as autism spectrum disorder and intellectual disability. However, the mechanism by which deficient NMD leads to neurodevelopmental dysfunction remains unknown, preventing development of targeted therapies. Here we identified novel protein-coding UPF2 (UP-Frameshift 2) variants in humans with NDD, including speech and language deficits. In parallel, we found that mice lacking Upf2 in the forebrain (Upf2 fb-KO mice) show impaired NMD, memory deficits, abnormal long-term potentiation (LTP), and social and communication deficits. Surprisingly, Upf2 fb-KO mice exhibit elevated expression of immune genes and brain inflammation. More importantly, treatment with two FDA-approved anti-inflammatory drugs reduced brain inflammation, restored LTP and long-term memory, and reversed social and communication deficits. Collectively, our findings indicate that impaired UPF2-dependent NMD leads to neurodevelopmental dysfunction and suggest that anti-inflammatory agents may prove effective for treatment of disorders with impaired NMD.

Original languageEnglish (US)
Pages (from-to)665-679.e8
JournalNeuron
Volume104
Issue number4
DOIs
StatePublished - Nov 20 2019
Externally publishedYes

Fingerprint

Long-Term Potentiation
Encephalitis
Anti-Inflammatory Agents
Communication
Long-Term Memory
Memory Disorders
Prosencephalon
Intellectual Disability
Language
Gene Expression
Pharmaceutical Preparations
Inhibition (Psychology)
Proteins
Neurodevelopmental Disorders
Therapeutics
Autism Spectrum Disorder

Keywords

  • autism
  • immune response
  • memory
  • mRNA quality control
  • neurodevelopmental disorders
  • speech disorder

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inhibition of Upf2-Dependent Nonsense-Mediated Decay Leads to Behavioral and Neurophysiological Abnormalities by Activating the Immune Response. / Johnson, Jennifer L.; Stoica, Loredana; Liu, Yuwei; Zhu, Ping Jun; Bhattacharya, Abhisek; Buffington, Shelly A.; Huq, Redwan; Eissa, N. Tony; Larsson, Ola; Porse, Bo T.; Domingo, Deepti; Nawaz, Urwah; Carroll, Renee; Jolly, Lachlan; Scerri, Tom S.; Kim, Hyung Goo; Brignell, Amanda; Coleman, Matthew J.; Braden, Ruth; Kini, Usha; Jackson, Victoria; Baxter, Anne; Bahlo, Melanie; Scheffer, Ingrid E.; Amor, David J.; Hildebrand, Michael S.; Bonnen, Penelope E.; Beeton, Christine; Gecz, Jozef; Morgan, Angela T.; Costa-Mattioli, Mauro.

In: Neuron, Vol. 104, No. 4, 20.11.2019, p. 665-679.e8.

Research output: Contribution to journalArticle

Johnson, JL, Stoica, L, Liu, Y, Zhu, PJ, Bhattacharya, A, Buffington, SA, Huq, R, Eissa, NT, Larsson, O, Porse, BT, Domingo, D, Nawaz, U, Carroll, R, Jolly, L, Scerri, TS, Kim, HG, Brignell, A, Coleman, MJ, Braden, R, Kini, U, Jackson, V, Baxter, A, Bahlo, M, Scheffer, IE, Amor, DJ, Hildebrand, MS, Bonnen, PE, Beeton, C, Gecz, J, Morgan, AT & Costa-Mattioli, M 2019, 'Inhibition of Upf2-Dependent Nonsense-Mediated Decay Leads to Behavioral and Neurophysiological Abnormalities by Activating the Immune Response', Neuron, vol. 104, no. 4, pp. 665-679.e8. https://doi.org/10.1016/j.neuron.2019.08.027
Johnson, Jennifer L. ; Stoica, Loredana ; Liu, Yuwei ; Zhu, Ping Jun ; Bhattacharya, Abhisek ; Buffington, Shelly A. ; Huq, Redwan ; Eissa, N. Tony ; Larsson, Ola ; Porse, Bo T. ; Domingo, Deepti ; Nawaz, Urwah ; Carroll, Renee ; Jolly, Lachlan ; Scerri, Tom S. ; Kim, Hyung Goo ; Brignell, Amanda ; Coleman, Matthew J. ; Braden, Ruth ; Kini, Usha ; Jackson, Victoria ; Baxter, Anne ; Bahlo, Melanie ; Scheffer, Ingrid E. ; Amor, David J. ; Hildebrand, Michael S. ; Bonnen, Penelope E. ; Beeton, Christine ; Gecz, Jozef ; Morgan, Angela T. ; Costa-Mattioli, Mauro. / Inhibition of Upf2-Dependent Nonsense-Mediated Decay Leads to Behavioral and Neurophysiological Abnormalities by Activating the Immune Response. In: Neuron. 2019 ; Vol. 104, No. 4. pp. 665-679.e8.
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AU - Huq, Redwan

AU - Eissa, N. Tony

AU - Larsson, Ola

AU - Porse, Bo T.

AU - Domingo, Deepti

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AU - Carroll, Renee

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