Inhibitory effect of glycyrrhizin on experimental pulmonary metastasis in mice inoculated with B16 melanoma

Makiko Kobayashi, Kazuhiko Fujita, Tatsushi Katakura, Tokuichiro Utsunomiya, Richard B. Pollard, Fujio Suzuki

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The pulmonary metastasis of B16 melanoma cells has been shown to be influenced by tumor-associated T helper type 2 cells (Th2 cells). Glycyrrhizin (GR), an active component of licorice roots, has been described as an inducer of CD4+ CD28+ TCRα/β+ T cells, which are capable of counteracting Th2 cytokine production by Th2 cells. In the present study, the anti-metastatic effect of GR in mice implanted with highly metastatic B16F10 melanoma cells was investigated. When mice inoculated with B16F10 melanoma cells (1 × 105 cells/mouse, i.v.) were treated with GR (10 mg/kg) 1, 3, 5 and 7 days after the tumor inoculation, the incidence of metastases decreased on average; 48 colonies were found in the GR-treated group and 208 in the saline-treated group. Similarly, 84% of metastases were inhibited in these mice after they were inoculated with splenic CD4+ T cells from mice treated with GR (GR-CD4+ T cells). These results suggest that GR inhibits the pulmonary metastases of B16 melanoma through the regulation of tumor-associated Th2 cells.

Original languageEnglish (US)
Pages (from-to)4053-4058
Number of pages6
JournalAnticancer Research
Volume22
Issue number6 C
StatePublished - Nov 1 2002

Keywords

  • B16 melanoma
  • Glycyrrhizin
  • Metastasis
  • Th2 cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Kobayashi, M., Fujita, K., Katakura, T., Utsunomiya, T., Pollard, R. B., & Suzuki, F. (2002). Inhibitory effect of glycyrrhizin on experimental pulmonary metastasis in mice inoculated with B16 melanoma. Anticancer Research, 22(6 C), 4053-4058.