Abstract
The pulmonary metastasis of B16 melanoma cells has been shown to be influenced by tumor-associated T helper type 2 cells (Th2 cells). Glycyrrhizin (GR), an active component of licorice roots, has been described as an inducer of CD4+ CD28+ TCRα/β+ T cells, which are capable of counteracting Th2 cytokine production by Th2 cells. In the present study, the anti-metastatic effect of GR in mice implanted with highly metastatic B16F10 melanoma cells was investigated. When mice inoculated with B16F10 melanoma cells (1 × 105 cells/mouse, i.v.) were treated with GR (10 mg/kg) 1, 3, 5 and 7 days after the tumor inoculation, the incidence of metastases decreased on average; 48 colonies were found in the GR-treated group and 208 in the saline-treated group. Similarly, 84% of metastases were inhibited in these mice after they were inoculated with splenic CD4+ T cells from mice treated with GR (GR-CD4+ T cells). These results suggest that GR inhibits the pulmonary metastases of B16 melanoma through the regulation of tumor-associated Th2 cells.
| Original language | English |
|---|---|
| Pages (from-to) | 4053-4058 |
| Number of pages | 6 |
| Journal | Anticancer Research |
| Volume | 22 |
| Issue number | 6 C |
| State | Published - Nov 2002 |
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Keywords
- B16 melanoma
- Glycyrrhizin
- Metastasis
- Th2 cells
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Inhibitory effect of glycyrrhizin on experimental pulmonary metastasis in mice inoculated with B16 melanoma. / Kobayashi, Makiko; Fujita, Kazuhiko; Katakura, Tatsushi; Utsunomiya, Tokuichiro; Pollard, Richard B.; Suzuki, Fujio.
In: Anticancer Research, Vol. 22, No. 6 C, 11.2002, p. 4053-4058.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibitory effect of glycyrrhizin on experimental pulmonary metastasis in mice inoculated with B16 melanoma
AU - Kobayashi, Makiko
AU - Fujita, Kazuhiko
AU - Katakura, Tatsushi
AU - Utsunomiya, Tokuichiro
AU - Pollard, Richard B.
AU - Suzuki, Fujio
PY - 2002/11
Y1 - 2002/11
N2 - The pulmonary metastasis of B16 melanoma cells has been shown to be influenced by tumor-associated T helper type 2 cells (Th2 cells). Glycyrrhizin (GR), an active component of licorice roots, has been described as an inducer of CD4+ CD28+ TCRα/β+ T cells, which are capable of counteracting Th2 cytokine production by Th2 cells. In the present study, the anti-metastatic effect of GR in mice implanted with highly metastatic B16F10 melanoma cells was investigated. When mice inoculated with B16F10 melanoma cells (1 × 105 cells/mouse, i.v.) were treated with GR (10 mg/kg) 1, 3, 5 and 7 days after the tumor inoculation, the incidence of metastases decreased on average; 48 colonies were found in the GR-treated group and 208 in the saline-treated group. Similarly, 84% of metastases were inhibited in these mice after they were inoculated with splenic CD4+ T cells from mice treated with GR (GR-CD4+ T cells). These results suggest that GR inhibits the pulmonary metastases of B16 melanoma through the regulation of tumor-associated Th2 cells.
AB - The pulmonary metastasis of B16 melanoma cells has been shown to be influenced by tumor-associated T helper type 2 cells (Th2 cells). Glycyrrhizin (GR), an active component of licorice roots, has been described as an inducer of CD4+ CD28+ TCRα/β+ T cells, which are capable of counteracting Th2 cytokine production by Th2 cells. In the present study, the anti-metastatic effect of GR in mice implanted with highly metastatic B16F10 melanoma cells was investigated. When mice inoculated with B16F10 melanoma cells (1 × 105 cells/mouse, i.v.) were treated with GR (10 mg/kg) 1, 3, 5 and 7 days after the tumor inoculation, the incidence of metastases decreased on average; 48 colonies were found in the GR-treated group and 208 in the saline-treated group. Similarly, 84% of metastases were inhibited in these mice after they were inoculated with splenic CD4+ T cells from mice treated with GR (GR-CD4+ T cells). These results suggest that GR inhibits the pulmonary metastases of B16 melanoma through the regulation of tumor-associated Th2 cells.
KW - B16 melanoma
KW - Glycyrrhizin
KW - Metastasis
KW - Th2 cells
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UR - http://www.scopus.com/inward/citedby.url?scp=0036875843&partnerID=8YFLogxK
M3 - Article
VL - 22
SP - 4053
EP - 4058
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 6 C
ER -