Inhibitory effect of ibuprofen on the cholera toxin-induced cyclic AMP response in Chinese hamster ovary cells

Johnny W. Peterson, Laura G. Ochoa, William D. Berg

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Ibuprofen, an inhibitor of prostaglandin synthesis in eukaryotic cells, was shown to inhibit the accumulation of 3′,5′-cyclic adenosine monophosphate (cyclic AMP) in Chinese hamster ovary (CHO) cells exposed to cholera toxin. The inhibition was dose dependent, with a dose of 100 μg/ml reducing the cholera toxin response by approximately 50%, and maximal inhibition was observed when the drug was applied to the cells simulataneously with or 1 h before the toxin. Although ibuprofen also inhibited adenylate cyclase stimulation by forskolin, suggesting a nonspecific effect, the drug had no effect on cholera toxin-induced cyclic AMP accumulation when added to the culture medium 15 min or more after the toxin.

Original languageEnglish (US)
Pages (from-to)139-143
Number of pages5
JournalFEMS Microbiology Letters
Volume56
Issue number2
DOIs
StatePublished - Dec 1 1988

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Keywords

  • Cholera toxin
  • Ibuprofen

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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