Initial sequence characterization of the rhabdoviruses of squamate reptiles, including a novel rhabdovirus from a caiman lizard (Dracaena guianensis)

James F X Wellehan, Allan P. Pessier, Linda L. Archer, April L. Childress, Elliott R. Jacobson, Robert B. Tesh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Rhabdoviruses infect a variety of hosts, including non-avian reptiles. Consensus PCR techniques were used to obtain partial RNA-dependent RNA polymerase gene sequence from five rhabdoviruses of South American lizards; Marco, Chaco, Timbo, Sena Madureira, and a rhabdovirus from a caiman lizard (Dracaena guianensis). The caiman lizard rhabdovirus formed inclusions in erythrocytes, which may be a route for infecting hematophagous insects. This is the first information on behavior of a rhabdovirus in squamates. We also obtained sequence from two rhabdoviruses of Australian lizards, confirming previous Charleville virus sequence and finding that, unlike a previous sequence report but in agreement with serologic reports, Almpiwar virus is clearly distinct from Charleville virus. Bayesian and maximum likelihood phylogenetic analysis revealed that most known rhabdoviruses of squamates cluster in the Almpiwar subgroup. The exception is Marco virus, which is found in the Hart Park group.

Original languageEnglish (US)
Pages (from-to)274-279
Number of pages6
JournalVeterinary Microbiology
Volume158
Issue number3-4
DOIs
StatePublished - Aug 17 2012

Fingerprint

Dracaena
Rhabdoviridae
Caiman
Alligators and Crocodiles
Reptiles
Lizards
Squamata
reptiles
lizards
Viruses
viruses
Erythrocyte Inclusions
RNA-directed RNA polymerase
hematophagous insects
RNA Replicase
Insects
Consensus
erythrocytes
nucleotide sequences
Polymerase Chain Reaction

Keywords

  • Chaco virus
  • Dracaena guianensis
  • Marco virus
  • Phylogeny
  • Rhabdoviridae
  • Sena Madureira virus
  • Timbo virus

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Initial sequence characterization of the rhabdoviruses of squamate reptiles, including a novel rhabdovirus from a caiman lizard (Dracaena guianensis). / Wellehan, James F X; Pessier, Allan P.; Archer, Linda L.; Childress, April L.; Jacobson, Elliott R.; Tesh, Robert B.

In: Veterinary Microbiology, Vol. 158, No. 3-4, 17.08.2012, p. 274-279.

Research output: Contribution to journalArticle

Wellehan, James F X ; Pessier, Allan P. ; Archer, Linda L. ; Childress, April L. ; Jacobson, Elliott R. ; Tesh, Robert B. / Initial sequence characterization of the rhabdoviruses of squamate reptiles, including a novel rhabdovirus from a caiman lizard (Dracaena guianensis). In: Veterinary Microbiology. 2012 ; Vol. 158, No. 3-4. pp. 274-279.
@article{647a3707a1d141218c273e692c499461,
title = "Initial sequence characterization of the rhabdoviruses of squamate reptiles, including a novel rhabdovirus from a caiman lizard (Dracaena guianensis)",
abstract = "Rhabdoviruses infect a variety of hosts, including non-avian reptiles. Consensus PCR techniques were used to obtain partial RNA-dependent RNA polymerase gene sequence from five rhabdoviruses of South American lizards; Marco, Chaco, Timbo, Sena Madureira, and a rhabdovirus from a caiman lizard (Dracaena guianensis). The caiman lizard rhabdovirus formed inclusions in erythrocytes, which may be a route for infecting hematophagous insects. This is the first information on behavior of a rhabdovirus in squamates. We also obtained sequence from two rhabdoviruses of Australian lizards, confirming previous Charleville virus sequence and finding that, unlike a previous sequence report but in agreement with serologic reports, Almpiwar virus is clearly distinct from Charleville virus. Bayesian and maximum likelihood phylogenetic analysis revealed that most known rhabdoviruses of squamates cluster in the Almpiwar subgroup. The exception is Marco virus, which is found in the Hart Park group.",
keywords = "Chaco virus, Dracaena guianensis, Marco virus, Phylogeny, Rhabdoviridae, Sena Madureira virus, Timbo virus",
author = "Wellehan, {James F X} and Pessier, {Allan P.} and Archer, {Linda L.} and Childress, {April L.} and Jacobson, {Elliott R.} and Tesh, {Robert B.}",
year = "2012",
month = "8",
day = "17",
doi = "10.1016/j.vetmic.2012.02.020",
language = "English (US)",
volume = "158",
pages = "274--279",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "3-4",

}

TY - JOUR

T1 - Initial sequence characterization of the rhabdoviruses of squamate reptiles, including a novel rhabdovirus from a caiman lizard (Dracaena guianensis)

AU - Wellehan, James F X

AU - Pessier, Allan P.

AU - Archer, Linda L.

AU - Childress, April L.

AU - Jacobson, Elliott R.

AU - Tesh, Robert B.

PY - 2012/8/17

Y1 - 2012/8/17

N2 - Rhabdoviruses infect a variety of hosts, including non-avian reptiles. Consensus PCR techniques were used to obtain partial RNA-dependent RNA polymerase gene sequence from five rhabdoviruses of South American lizards; Marco, Chaco, Timbo, Sena Madureira, and a rhabdovirus from a caiman lizard (Dracaena guianensis). The caiman lizard rhabdovirus formed inclusions in erythrocytes, which may be a route for infecting hematophagous insects. This is the first information on behavior of a rhabdovirus in squamates. We also obtained sequence from two rhabdoviruses of Australian lizards, confirming previous Charleville virus sequence and finding that, unlike a previous sequence report but in agreement with serologic reports, Almpiwar virus is clearly distinct from Charleville virus. Bayesian and maximum likelihood phylogenetic analysis revealed that most known rhabdoviruses of squamates cluster in the Almpiwar subgroup. The exception is Marco virus, which is found in the Hart Park group.

AB - Rhabdoviruses infect a variety of hosts, including non-avian reptiles. Consensus PCR techniques were used to obtain partial RNA-dependent RNA polymerase gene sequence from five rhabdoviruses of South American lizards; Marco, Chaco, Timbo, Sena Madureira, and a rhabdovirus from a caiman lizard (Dracaena guianensis). The caiman lizard rhabdovirus formed inclusions in erythrocytes, which may be a route for infecting hematophagous insects. This is the first information on behavior of a rhabdovirus in squamates. We also obtained sequence from two rhabdoviruses of Australian lizards, confirming previous Charleville virus sequence and finding that, unlike a previous sequence report but in agreement with serologic reports, Almpiwar virus is clearly distinct from Charleville virus. Bayesian and maximum likelihood phylogenetic analysis revealed that most known rhabdoviruses of squamates cluster in the Almpiwar subgroup. The exception is Marco virus, which is found in the Hart Park group.

KW - Chaco virus

KW - Dracaena guianensis

KW - Marco virus

KW - Phylogeny

KW - Rhabdoviridae

KW - Sena Madureira virus

KW - Timbo virus

UR - http://www.scopus.com/inward/record.url?scp=84862514781&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862514781&partnerID=8YFLogxK

U2 - 10.1016/j.vetmic.2012.02.020

DO - 10.1016/j.vetmic.2012.02.020

M3 - Article

VL - 158

SP - 274

EP - 279

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -