Immunogenicity of the protein recombinant ASP1r of a caninum in a murine model

Translated title of the contribution: Inmunogenicidad de la proteína recombinante ASP1r de Ancylostoma caninum en un modelo murino

Maria Giraldo Giraldo, O. Jhon C. Castaño

Research output: Contribution to journalArticle

Abstract

Objective. To construct a recombinant plasmid expressing the ASP1r protein of A. caninum and evaluate its immunogenic capacity in a murine model. Materials and methods. RNA of adults of Ancylostoma caninum was amplified by reverse transcription polymerase chain reaction. The ASP1 protein gene was inserted into the pcDNA3 vector. Plasmid was digested with Bamh1 and EcoR1 and cloning was performed directionally. Later a transformation and selection of E. coli DH5a cell competent with the product for the ligation was made. Then, a screening by PCR was carried out to confirm the presence of ASP1 gene. PcDNA3-ASP1 was inoculated by intraglandular parotide and intramuscular route in Balb/c mice. Antibodies in these animals was measured in serum and saliva by ELISA and immunochemistry. Results. PcDNA3-ASP1 was incorporated and expressed in E. coli DH5a cell. This recombinant plásmid was able to produce antibodies anti-ASP1 specific in Balb/c mice. Discussion. It was possible to demonstrate that using of pcDNA3-ASP1 did not display reactogenicity and it did not produce unfavorable reactions, futhermore, it induced a humoral response against the excreción/secretion protein of Ancylostoma caninum in mice.

Original languageSpanish
Pages (from-to)1667-1676
Number of pages10
JournalRevista MVZ Cordoba
Volume14
Issue number2
StatePublished - Jan 1 2009
Externally publishedYes

Fingerprint

Ancylostoma
Ancylostoma caninum
plasmid
antibody
protein
plasmids
mice
Plasmids
immunochemistry
Escherichia coli
Immunochemistry
Polymerase Chain Reaction
saliva
protein secretion
antibodies
gene
Staphylococcal Protein A
Saliva
humoral immunity
secretion

Keywords

  • Ancylostoma caninum
  • ASP1 protein
  • Cloning molecular
  • DNA vaccines
  • Enzyme-linked immunosorbent assay
  • Immunohistochemistry

ASJC Scopus subject areas

  • Aquatic Science
  • Animal Science and Zoology
  • veterinary(all)

Cite this

Immunogenicity of the protein recombinant ASP1r of a caninum in a murine model. / Giraldo Giraldo, Maria; Castaño, O. Jhon C.

In: Revista MVZ Cordoba, Vol. 14, No. 2, 01.01.2009, p. 1667-1676.

Research output: Contribution to journalArticle

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