Inosine inhibits inflammatory cytokine production by a posttranscriptional mechanism and protects against endotoxin-induced shock

György Haskó, David G. Kuhel, Zoltán H. Németh, Jon G. Mabley, Robert F. Stachlewitz, László Virág, Zsolt Lohinai, Garry J. Southan, Andrew L. Salzman, Csaba Szabo

Research output: Contribution to journalArticle

201 Citations (Scopus)

Abstract

Extracellular purines, including adenosine and ATP, are potent endogenous immunomodulatory molecules. Inosine, a degradation product of these purines, can reach high concentrations in the extracellular space under conditions associated with cellular metabolic stress such as inflammation or ischemia. In the present study, we investigated whether extracellular inosine can affect inflammatory/immune processes. In immunostimulated macrophages and spleen cells, inosine potently inhibited the production of the proinflammatory cytokines TNF-α, IL-1, IL-12, macrophage-inflammatory protein-1α, and IFN-γ, but failed to alter the production of the anti- inflammatory cytokine IL-10. The effect of inosine did not require cellular uptake by nucleoside transporters and was partially reversed by blockade of adenosine A1 and A2 receptors. Inosine inhibited cytokine production by a posttranscriptional mechanism. The activity of inosine was independent of activation of the p38 and p42/p44 mitogen-activated protein kinases, the phosphorylation of the c-Jun terminal kinase, the degradation of inhibitory factor κB, and elevation of intracellular cAMP. Inosine suppressed proinflammatory cytokine production and mortality in a mouse endotoxemic model. Taken together, inosine has multiple anti-inflammatory effects. These findings, coupled with the fact that inosine has very low toxicity, suggest that this agent may be useful in the treatment of inflammatory/ischemic diseases.

Original languageEnglish (US)
Pages (from-to)1013-1019
Number of pages7
JournalJournal of Immunology
Volume164
Issue number2
StatePublished - Jan 15 2000
Externally publishedYes

Fingerprint

Inosine
Endotoxins
Shock
Cytokines
Purines
Anti-Inflammatory Agents
Adenosine A2 Receptors
Nucleoside Transport Proteins
Adenosine A1 Receptors
Macrophage Inflammatory Proteins
Physiological Stress
Mitogen-Activated Protein Kinase 1
Extracellular Space
Interleukin-12
Interleukin-1
Interleukin-10
Adenosine
Phosphotransferases
Spleen
Ischemia

ASJC Scopus subject areas

  • Immunology

Cite this

Haskó, G., Kuhel, D. G., Németh, Z. H., Mabley, J. G., Stachlewitz, R. F., Virág, L., ... Szabo, C. (2000). Inosine inhibits inflammatory cytokine production by a posttranscriptional mechanism and protects against endotoxin-induced shock. Journal of Immunology, 164(2), 1013-1019.

Inosine inhibits inflammatory cytokine production by a posttranscriptional mechanism and protects against endotoxin-induced shock. / Haskó, György; Kuhel, David G.; Németh, Zoltán H.; Mabley, Jon G.; Stachlewitz, Robert F.; Virág, László; Lohinai, Zsolt; Southan, Garry J.; Salzman, Andrew L.; Szabo, Csaba.

In: Journal of Immunology, Vol. 164, No. 2, 15.01.2000, p. 1013-1019.

Research output: Contribution to journalArticle

Haskó, G, Kuhel, DG, Németh, ZH, Mabley, JG, Stachlewitz, RF, Virág, L, Lohinai, Z, Southan, GJ, Salzman, AL & Szabo, C 2000, 'Inosine inhibits inflammatory cytokine production by a posttranscriptional mechanism and protects against endotoxin-induced shock', Journal of Immunology, vol. 164, no. 2, pp. 1013-1019.
Haskó G, Kuhel DG, Németh ZH, Mabley JG, Stachlewitz RF, Virág L et al. Inosine inhibits inflammatory cytokine production by a posttranscriptional mechanism and protects against endotoxin-induced shock. Journal of Immunology. 2000 Jan 15;164(2):1013-1019.
Haskó, György ; Kuhel, David G. ; Németh, Zoltán H. ; Mabley, Jon G. ; Stachlewitz, Robert F. ; Virág, László ; Lohinai, Zsolt ; Southan, Garry J. ; Salzman, Andrew L. ; Szabo, Csaba. / Inosine inhibits inflammatory cytokine production by a posttranscriptional mechanism and protects against endotoxin-induced shock. In: Journal of Immunology. 2000 ; Vol. 164, No. 2. pp. 1013-1019.
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