Abstract
Inosine, a naturally occurring purine formed from the breakdown of adenosine, has recently been shown to exert powerful anti-inflammatory effects both in vivo and in vitro. This study evaluated inosine as a potential therapy for colitis. Colitis was induced in mice by the administration of dextran sulfate sodium (DSS). Oral treatment with inosine was begun either before the onset of colitis or as a posttreatment once colitis was established. Evaluation of colon damage and inflammation was determined grossly (body wt, rectal bleeding), histologically, and biochemically (colon levels of MPO, MDA, and cytokines). DSS-induced colitis significantly increased inflammatory cell infiltration into the colon. DSS-induced colitis also increased colon levels of lipid peroxidation, cytokines, and chemokines. Inosine protected the colon from DSS-induced inflammatory cell infiltration and lipid peroxidation. Inosine also partially reduced these parameters in an experimental model of established colitis. Thus inosine treatment may be a potential therapy in colitis.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Gastrointestinal and Liver Physiology |
| Volume | 284 |
| Issue number | 1 47-1 |
| State | Published - Jan 1 2003 |
| Externally published | Yes |
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Keywords
- Colon
- Cytokines
- Dextran sodium sulfate
- Purine
ASJC Scopus subject areas
- Gastroenterology
- Physiology
Cite this
Inosine reduces inflammation and improves survival in a murine model of colitis. / Mabley, J. G.; Pacher, P.; Liaudet, L.; Soriano, F. G.; Hasko, G.; Marton, A.; Szabo, Csaba; Salzman, A. L.
In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 284, No. 1 47-1, 01.01.2003.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inosine reduces inflammation and improves survival in a murine model of colitis
AU - Mabley, J. G.
AU - Pacher, P.
AU - Liaudet, L.
AU - Soriano, F. G.
AU - Hasko, G.
AU - Marton, A.
AU - Szabo, Csaba
AU - Salzman, A. L.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Inosine, a naturally occurring purine formed from the breakdown of adenosine, has recently been shown to exert powerful anti-inflammatory effects both in vivo and in vitro. This study evaluated inosine as a potential therapy for colitis. Colitis was induced in mice by the administration of dextran sulfate sodium (DSS). Oral treatment with inosine was begun either before the onset of colitis or as a posttreatment once colitis was established. Evaluation of colon damage and inflammation was determined grossly (body wt, rectal bleeding), histologically, and biochemically (colon levels of MPO, MDA, and cytokines). DSS-induced colitis significantly increased inflammatory cell infiltration into the colon. DSS-induced colitis also increased colon levels of lipid peroxidation, cytokines, and chemokines. Inosine protected the colon from DSS-induced inflammatory cell infiltration and lipid peroxidation. Inosine also partially reduced these parameters in an experimental model of established colitis. Thus inosine treatment may be a potential therapy in colitis.
AB - Inosine, a naturally occurring purine formed from the breakdown of adenosine, has recently been shown to exert powerful anti-inflammatory effects both in vivo and in vitro. This study evaluated inosine as a potential therapy for colitis. Colitis was induced in mice by the administration of dextran sulfate sodium (DSS). Oral treatment with inosine was begun either before the onset of colitis or as a posttreatment once colitis was established. Evaluation of colon damage and inflammation was determined grossly (body wt, rectal bleeding), histologically, and biochemically (colon levels of MPO, MDA, and cytokines). DSS-induced colitis significantly increased inflammatory cell infiltration into the colon. DSS-induced colitis also increased colon levels of lipid peroxidation, cytokines, and chemokines. Inosine protected the colon from DSS-induced inflammatory cell infiltration and lipid peroxidation. Inosine also partially reduced these parameters in an experimental model of established colitis. Thus inosine treatment may be a potential therapy in colitis.
KW - Colon
KW - Cytokines
KW - Dextran sodium sulfate
KW - Purine
UR - http://www.scopus.com/inward/record.url?scp=0037230080&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037230080&partnerID=8YFLogxK
M3 - Article
C2 - 12388199
AN - SCOPUS:0037230080
VL - 284
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
SN - 0193-1849
IS - 1 47-1
ER -