Insect-specific viruses detected in laboratory mosquito colonies and their potential implications for experiments evaluating arbovirus vector competence

Bethany G. Bolling, Nikos Vasilakis, Hilda Guzman, Steven Widen, Thomas Wood, Vsevolod Popov, Saravanan Thangamani, Robert B. Tesh

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Recently, there has been a dramatic increase in the detection and characterization of insect-specific viruses in field-collected mosquitoes. Evidence suggests that these viruses are ubiquitous in nature and that many are maintained by vertical transmission in mosquito populations. Some studies suggest that the presence of insect-specific viruses may inhibit replication of a super-infecting arbovirus, thus altering vector competence of the mosquito host. Accordingly, we screened our laboratory mosquito colonies for insect-specific viruses. Pools of colony mosquitoes were homogenized and inoculated into cultures of Aedes albopictus (C6/36) cells. The infected cells were examined by electron microscopy and deep sequencing was performed on RNA extracts. Electron micrograph images indicated the presence of three different viruses in three of our laboratory mosquito colonies. Potential implications of these findings for vector competence studies are discussed.

Original languageEnglish (US)
Pages (from-to)422-428
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume92
Issue number2
DOIs
StatePublished - Feb 1 2015

Fingerprint

Insect Viruses
Arboviruses
Culicidae
Mental Competency
Viruses
High-Throughput Nucleotide Sequencing
Aedes
Electron Microscopy
RNA
Electrons
Population

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Insect-specific viruses detected in laboratory mosquito colonies and their potential implications for experiments evaluating arbovirus vector competence. / Bolling, Bethany G.; Vasilakis, Nikos; Guzman, Hilda; Widen, Steven; Wood, Thomas; Popov, Vsevolod; Thangamani, Saravanan; Tesh, Robert B.

In: American Journal of Tropical Medicine and Hygiene, Vol. 92, No. 2, 01.02.2015, p. 422-428.

Research output: Contribution to journalArticle

@article{789ab4df099a4e23aa922f3cc2cf64bf,
title = "Insect-specific viruses detected in laboratory mosquito colonies and their potential implications for experiments evaluating arbovirus vector competence",
abstract = "Recently, there has been a dramatic increase in the detection and characterization of insect-specific viruses in field-collected mosquitoes. Evidence suggests that these viruses are ubiquitous in nature and that many are maintained by vertical transmission in mosquito populations. Some studies suggest that the presence of insect-specific viruses may inhibit replication of a super-infecting arbovirus, thus altering vector competence of the mosquito host. Accordingly, we screened our laboratory mosquito colonies for insect-specific viruses. Pools of colony mosquitoes were homogenized and inoculated into cultures of Aedes albopictus (C6/36) cells. The infected cells were examined by electron microscopy and deep sequencing was performed on RNA extracts. Electron micrograph images indicated the presence of three different viruses in three of our laboratory mosquito colonies. Potential implications of these findings for vector competence studies are discussed.",
author = "Bolling, {Bethany G.} and Nikos Vasilakis and Hilda Guzman and Steven Widen and Thomas Wood and Vsevolod Popov and Saravanan Thangamani and Tesh, {Robert B.}",
year = "2015",
month = "2",
day = "1",
doi = "10.4269/ajtmh.14-0330",
language = "English (US)",
volume = "92",
pages = "422--428",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "2",

}

TY - JOUR

T1 - Insect-specific viruses detected in laboratory mosquito colonies and their potential implications for experiments evaluating arbovirus vector competence

AU - Bolling, Bethany G.

AU - Vasilakis, Nikos

AU - Guzman, Hilda

AU - Widen, Steven

AU - Wood, Thomas

AU - Popov, Vsevolod

AU - Thangamani, Saravanan

AU - Tesh, Robert B.

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Recently, there has been a dramatic increase in the detection and characterization of insect-specific viruses in field-collected mosquitoes. Evidence suggests that these viruses are ubiquitous in nature and that many are maintained by vertical transmission in mosquito populations. Some studies suggest that the presence of insect-specific viruses may inhibit replication of a super-infecting arbovirus, thus altering vector competence of the mosquito host. Accordingly, we screened our laboratory mosquito colonies for insect-specific viruses. Pools of colony mosquitoes were homogenized and inoculated into cultures of Aedes albopictus (C6/36) cells. The infected cells were examined by electron microscopy and deep sequencing was performed on RNA extracts. Electron micrograph images indicated the presence of three different viruses in three of our laboratory mosquito colonies. Potential implications of these findings for vector competence studies are discussed.

AB - Recently, there has been a dramatic increase in the detection and characterization of insect-specific viruses in field-collected mosquitoes. Evidence suggests that these viruses are ubiquitous in nature and that many are maintained by vertical transmission in mosquito populations. Some studies suggest that the presence of insect-specific viruses may inhibit replication of a super-infecting arbovirus, thus altering vector competence of the mosquito host. Accordingly, we screened our laboratory mosquito colonies for insect-specific viruses. Pools of colony mosquitoes were homogenized and inoculated into cultures of Aedes albopictus (C6/36) cells. The infected cells were examined by electron microscopy and deep sequencing was performed on RNA extracts. Electron micrograph images indicated the presence of three different viruses in three of our laboratory mosquito colonies. Potential implications of these findings for vector competence studies are discussed.

UR - http://www.scopus.com/inward/record.url?scp=84922326531&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922326531&partnerID=8YFLogxK

U2 - 10.4269/ajtmh.14-0330

DO - 10.4269/ajtmh.14-0330

M3 - Article

VL - 92

SP - 422

EP - 428

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 2

ER -