Insight into DNA and protein transport in double-stranded DNA viruses

The structure of bacteriophage N4

Kyung Choi, J. McPartland, I. Kaganman, V. D. Bowman, L. B. Rothman-Denes, M. G. Rossmann

Research output: Contribution to journalArticle

Abstract

N4 is a lytic double-stranded DNA (dsDNA) bacteriophage that infects the Escherichia coli K12 strains. This article reports the structure of this bacteriophage as determined to 14-Å resolution by cryoelectron microscopy (Fig. 1), The identity and placement of the different parts of the virion in the three-dimensional structure was confirmed by a combination of bioinformatics and analysis of the difference density maps of the wild type (WT) and mutants lacking some virion gene products. The virion has an icosahedral head housing the vRNAP, the viral RNA polymerase, and the DNA. The head is supported by a neck composed of 12 subunits forming a pentameric base. The tail assembly is formed by a hollow cylindrical tube covered by a noncontractile sheath. The structure of the virion provides insight into the process of injection of material into the host cell. Briefly, the virion adsorbs onto the host's surface by the tail sheath and the materials within it, starting with the vRNAP, are injected into the host cell. The incompatibility of the vRNAP size with the small diameter of the tail suggests that vRNAP is released in a denatured or partially unfolded form that must be quickly renatured in the host cell's cytoplasm.

Original languageEnglish (US)
Pages (from-to)489-490
Number of pages2
JournalChemtracts
Volume20
Issue number12
StatePublished - 2008
Externally publishedYes

Fingerprint

Bacteriophage N4
Viral Structures
Bacteriophages
Protein Transport
Viruses
Virion
Viral RNA
DNA-Directed RNA Polymerases
Bioinformatics
Escherichia coli
Microscopic examination
Proteins
Genes
Head
Cryoelectron Microscopy
Escherichia coli K12
Computational Biology
Cytoplasm
Neck
Injections

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry
  • Molecular Biology

Cite this

Choi, K., McPartland, J., Kaganman, I., Bowman, V. D., Rothman-Denes, L. B., & Rossmann, M. G. (2008). Insight into DNA and protein transport in double-stranded DNA viruses: The structure of bacteriophage N4. Chemtracts, 20(12), 489-490.

Insight into DNA and protein transport in double-stranded DNA viruses : The structure of bacteriophage N4. / Choi, Kyung; McPartland, J.; Kaganman, I.; Bowman, V. D.; Rothman-Denes, L. B.; Rossmann, M. G.

In: Chemtracts, Vol. 20, No. 12, 2008, p. 489-490.

Research output: Contribution to journalArticle

Choi, K, McPartland, J, Kaganman, I, Bowman, VD, Rothman-Denes, LB & Rossmann, MG 2008, 'Insight into DNA and protein transport in double-stranded DNA viruses: The structure of bacteriophage N4', Chemtracts, vol. 20, no. 12, pp. 489-490.
Choi K, McPartland J, Kaganman I, Bowman VD, Rothman-Denes LB, Rossmann MG. Insight into DNA and protein transport in double-stranded DNA viruses: The structure of bacteriophage N4. Chemtracts. 2008;20(12):489-490.
Choi, Kyung ; McPartland, J. ; Kaganman, I. ; Bowman, V. D. ; Rothman-Denes, L. B. ; Rossmann, M. G. / Insight into DNA and protein transport in double-stranded DNA viruses : The structure of bacteriophage N4. In: Chemtracts. 2008 ; Vol. 20, No. 12. pp. 489-490.
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