Insights into Reston virus spillovers and adaption from virus whole genome sequences

César G. Albariño, Lisa Wiggleton Guerrero, Harley M. Jenks, Ayan K. Chakrabarti, Thomas Ksiazek, Pierre E. Rollin, Stuart T. Nichol

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Reston virus (family Filoviridae) is unique among the viruses of the Ebolavirus genus in that it is considered non-pathogenic in humans, in contrast to the other members which are highly virulent. The virus has however, been associated with several outbreaks of highly lethal hemorrhagic fever in non-human primates (NHPs), specifically cynomolgus monkeys (Macaca fascicularis) originating in the Philippines. In addition, Reston virus has been isolated from domestic pigs in the Philippines. To better understand virus spillover events and potential adaption to new hosts, the whole genome sequences of representative Reston virus isolates were obtained using a next generation sequencing (NGS) approach and comparative genomic analysis and virus fitness analyses were performed. Nine virus genome sequences were completed for novel and previously described isolates obtained from a variety of hosts including a human case, non-human primates and pigs. Results of phylogenetic analysis of the sequence differences are consistent with multiple independent introductions of RESTV from a still unknown natural reservoir into non-human primates and swine farming operations. No consistent virus genetic markers were found specific for viruses associated with primate or pig infections, but similar to what had been seen with some Ebola viruses detected in the large Western Africa outbreak in 2014-2016, a truncated version of VP30 was identified in a subgroup of Reston viruses obtained from an outbreak in pigs 2008-2009. Finally, the genetic comparison of two closely related viruses, one isolated from a human case and one from an NHP, showed amino acid differences in the viral polymerase and detectable differences were found in competitive growth assays on human and NHP cell lines.

Original languageEnglish (US)
Article numbere0178224
JournalPLoS One
Volume12
Issue number5
DOIs
StatePublished - May 1 2017

Fingerprint

Viruses
Genes
Genome
viruses
genome
Primates
Swine
Ebolavirus
swine
Disease Outbreaks
Philippines
Macaca fascicularis
Filoviridae
Reston
Sus scrofa
Western Africa
Agriculture
lethal genes
Genetic Markers
fever

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Albariño, C. G., Guerrero, L. W., Jenks, H. M., Chakrabarti, A. K., Ksiazek, T., Rollin, P. E., & Nichol, S. T. (2017). Insights into Reston virus spillovers and adaption from virus whole genome sequences. PLoS One, 12(5), [e0178224]. https://doi.org/10.1371/journal.pone.0178224

Insights into Reston virus spillovers and adaption from virus whole genome sequences. / Albariño, César G.; Guerrero, Lisa Wiggleton; Jenks, Harley M.; Chakrabarti, Ayan K.; Ksiazek, Thomas; Rollin, Pierre E.; Nichol, Stuart T.

In: PLoS One, Vol. 12, No. 5, e0178224, 01.05.2017.

Research output: Contribution to journalArticle

Albariño, CG, Guerrero, LW, Jenks, HM, Chakrabarti, AK, Ksiazek, T, Rollin, PE & Nichol, ST 2017, 'Insights into Reston virus spillovers and adaption from virus whole genome sequences', PLoS One, vol. 12, no. 5, e0178224. https://doi.org/10.1371/journal.pone.0178224
Albariño CG, Guerrero LW, Jenks HM, Chakrabarti AK, Ksiazek T, Rollin PE et al. Insights into Reston virus spillovers and adaption from virus whole genome sequences. PLoS One. 2017 May 1;12(5). e0178224. https://doi.org/10.1371/journal.pone.0178224
Albariño, César G. ; Guerrero, Lisa Wiggleton ; Jenks, Harley M. ; Chakrabarti, Ayan K. ; Ksiazek, Thomas ; Rollin, Pierre E. ; Nichol, Stuart T. / Insights into Reston virus spillovers and adaption from virus whole genome sequences. In: PLoS One. 2017 ; Vol. 12, No. 5.
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