Insights into the Prostanoid Pathway in the Ovary Development of the Penaeid Shrimp Penaeus monodon

Wananit Wimuttisuk, Punsa Tobwor, Pacharawan Deenarn, Kannawat Danwisetkanjana, Decha Pinkaew, Kanyawim Kirtikara, Vanicha Vichai

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The prostanoid pathway converts polyunsaturated fatty acids (PUFAs) into bioactive lipid mediators, including prostaglandins, thromboxanes and prostacyclins, all of which play vital roles in the immune and reproductive systems in most animal phyla. In crustaceans, PUFAs and prostaglandins have been detected and often associated with female reproductive maturation. However, the presence of prostanoid biosynthesis genes remained in question in these species. In this study, we outlined the prostanoid pathway in the black tiger shrimp Penaeus monodon based on the amplification of nine prostanoid biosynthesis genes: cytosolic phospholipase A2, hematopoietic prostaglandin D synthase, glutathione-dependent prostaglandin D synthase, prostaglandin E synthase 1, prostaglandin E synthase 2, prostaglandin E synthase 3, prostaglandin F synthase, thromboxane A synthase and cyclooxygenase. TBLASTX analysis confirmed the identities of these genes with 51-99% sequence identities to their closest homologs. In addition, prostaglandin F (PGF), which is a product of the prostaglandin F synthase enzyme, was detected for the first time in P. monodon ovaries along with the previously identified PUFAs and prostaglandin E2 (PGE2) using RP-HPLC and mass-spectrometry. The prostaglandin synthase activity was also observed in shrimp ovary homogenates using in vitro activity assay. When prostaglandin biosynthesis was examined in different stages of shrimp ovaries, we found that the amounts of prostaglandin F synthase gene transcripts and PGF decreased as the ovaries matured. These findings not only indicate the presence of a functional prostanoid pathway in penaeid shrimp, but also suggest a possible role of the PGF biosynthesis in shrimp ovarian development.

Original languageEnglish (US)
Article numbere76934
JournalPloS one
Volume8
Issue number10
DOIs
StatePublished - Oct 8 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Wimuttisuk, W., Tobwor, P., Deenarn, P., Danwisetkanjana, K., Pinkaew, D., Kirtikara, K., & Vichai, V. (2013). Insights into the Prostanoid Pathway in the Ovary Development of the Penaeid Shrimp Penaeus monodon. PloS one, 8(10), [e76934]. https://doi.org/10.1371/journal.pone.0076934