Insights into the structure and assembly of the bacteriophage φ29 double-stranded DNA packaging motor

Sheng Cao, Mitul Saha, Wei Zhao, Paul J. Jardine, Wei Zhang, Shelley Grimes, Marc Morais

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The tailed double-stranded DNA (dsDNA) bacteriophage φ29 packages its 19.3-kbp genome into a preassembled procapsid structure by using a transiently assembled phage-encoded molecular motor. This process is remarkable considering that compaction of DNA to near-crystalline densities within the confined space of the capsid requires that the packaging motor work against significant entropic, enthalpic, and DNA-bending energies. The motor consists of three phage-encoded components: the dodecameric connector protein gp10, an oligomeric RNA molecule known as the prohead RNA (pRNA), and the homomeric ring ATPase gp16. Although atomic resolution structures of the connector and different pRNA subdomains have been determined, the mechanism of self-assembly and the resulting stoichiometry of the various motor components on the phage capsid have been the subject of considerable controversy. Here a subnanometer asymmetric cryoelectron microscopy (cryo-EM) reconstruction of a connector-pRNA complex at a unique vertex of the procapsid conclusively demonstrates the pentameric symmetry of the pRNA and illuminates the relative arrangement of the connector and the pRNA. Additionally, a combination of biochemical and cryo-EM analyses of motor assembly intermediates suggests a sequence of molecular events that constitute the pathway by which the motor assembles on the head, thereby reconciling conflicting data regarding pRNA assembly and stoichiometry. Taken together, these data provide new insight into the assembly, structure, and mechanism of a complex molecular machine.

Original languageEnglish (US)
Pages (from-to)3986-3996
Number of pages11
JournalJournal of Virology
Volume88
Issue number8
DOIs
StatePublished - 2014

Fingerprint

Product Packaging
Bacteriophages
packaging
RNA
bacteriophages
Cryoelectron Microscopy
capsid
Capsid
stoichiometry
Confined Spaces
Efferent Pathways
virus assembly
adenosinetriphosphatase
Adenosine Triphosphatases
Head
Genome
genome
energy
Proteins
proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Insights into the structure and assembly of the bacteriophage φ29 double-stranded DNA packaging motor. / Cao, Sheng; Saha, Mitul; Zhao, Wei; Jardine, Paul J.; Zhang, Wei; Grimes, Shelley; Morais, Marc.

In: Journal of Virology, Vol. 88, No. 8, 2014, p. 3986-3996.

Research output: Contribution to journalArticle

Cao, Sheng ; Saha, Mitul ; Zhao, Wei ; Jardine, Paul J. ; Zhang, Wei ; Grimes, Shelley ; Morais, Marc. / Insights into the structure and assembly of the bacteriophage φ29 double-stranded DNA packaging motor. In: Journal of Virology. 2014 ; Vol. 88, No. 8. pp. 3986-3996.
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