Insulin activates guanosine 5′-[γ-thio] triphosphate (GTPγS) binding to a novel GTP-binding protein, GIR, from human placenta

Satish K. Srivastava; Ugra S. Singh

doi:10.1016/S0006-291X(05)80062-2

Insulin activates guanosine 5′-[γ-thio] triphosphate (GTPγS) binding to a novel GTP-binding protein, G_IR, from human placenta

Satish K. Srivastava
, Ugra S. Singh

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A novel GTP-binding protein, G_IR, along with insulin receptor (IR), has been partially purified from human placenta. A non-hydrolyzable substrate, GTPγS which is known to bind to GTP-binding proteins with high affinity, reduces insulin binding to IR-G_IR fraction by approximately 29% and 100 nM insulin stimulates GTPγS binding to IR-G_IR fraction by approximately five-fold. The molecular weight of the protein (may be subunit) that binds to 8-azido-GTP, a photoaffinity label for G-proteins, is approximately 66,000.

Original language	English (US)
Pages (from-to)	501-506
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	173
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(05)80062-2
State	Published - Dec 14 1990

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/S0006-291X(05)80062-2

Cite this

@article{d13749ef37804237bfc288490448ecba,

title = "Insulin activates guanosine 5′-[γ-thio] triphosphate (GTPγS) binding to a novel GTP-binding protein, GIR, from human placenta",

abstract = "A novel GTP-binding protein, GIR, along with insulin receptor (IR), has been partially purified from human placenta. A non-hydrolyzable substrate, GTPγS which is known to bind to GTP-binding proteins with high affinity, reduces insulin binding to IR-GIR fraction by approximately 29\% and 100 nM insulin stimulates GTPγS binding to IR-GIR fraction by approximately five-fold. The molecular weight of the protein (may be subunit) that binds to 8-azido-GTP, a photoaffinity label for G-proteins, is approximately 66,000.",

author = "Srivastava, \{Satish K.\} and Singh, \{Ugra S.\}",

note = "Funding Information: We gratefully acknowledge the valuable discussion with Dr. Charles A. Stuart, Department of Internal Medicine, UTMB. This work was supported in part by NIH Grants EY-01677 and DK-36118.",

year = "1990",

month = dec,

day = "14",

doi = "10.1016/S0006-291X(05)80062-2",

language = "English (US)",

volume = "173",

pages = "501--506",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Insulin activates guanosine 5′-[γ-thio] triphosphate (GTPγS) binding to a novel GTP-binding protein, GIR, from human placenta

AU - Srivastava, Satish K.

AU - Singh, Ugra S.

N1 - Funding Information: We gratefully acknowledge the valuable discussion with Dr. Charles A. Stuart, Department of Internal Medicine, UTMB. This work was supported in part by NIH Grants EY-01677 and DK-36118.

PY - 1990/12/14

Y1 - 1990/12/14

N2 - A novel GTP-binding protein, GIR, along with insulin receptor (IR), has been partially purified from human placenta. A non-hydrolyzable substrate, GTPγS which is known to bind to GTP-binding proteins with high affinity, reduces insulin binding to IR-GIR fraction by approximately 29% and 100 nM insulin stimulates GTPγS binding to IR-GIR fraction by approximately five-fold. The molecular weight of the protein (may be subunit) that binds to 8-azido-GTP, a photoaffinity label for G-proteins, is approximately 66,000.

AB - A novel GTP-binding protein, GIR, along with insulin receptor (IR), has been partially purified from human placenta. A non-hydrolyzable substrate, GTPγS which is known to bind to GTP-binding proteins with high affinity, reduces insulin binding to IR-GIR fraction by approximately 29% and 100 nM insulin stimulates GTPγS binding to IR-GIR fraction by approximately five-fold. The molecular weight of the protein (may be subunit) that binds to 8-azido-GTP, a photoaffinity label for G-proteins, is approximately 66,000.

UR - https://www.scopus.com/pages/publications/0025685604

UR - https://www.scopus.com/pages/publications/0025685604#tab=citedBy

U2 - 10.1016/S0006-291X(05)80062-2

DO - 10.1016/S0006-291X(05)80062-2

M3 - Article

C2 - 2124484

AN - SCOPUS:0025685604

SN - 0006-291X

VL - 173

SP - 501

EP - 506

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Insulin activates guanosine 5′-[γ-thio] triphosphate (GTPγS) binding to a novel GTP-binding protein, G_IR, from human placenta

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this