Insulin attenuates the cytokine response in a burn wound infection model

Rene Przkora, David Herndon, Celeste Finnerty, Marc G. Jeschke

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A massive burn is one of the most serious injuries resulting in major imbalances of the immune system. The aftermath of a burn is frequently complicated by infections and septic events that additionally increase mortality and morbidity.The aim of the present study was to investigate if insulin attenuates the cytokine response of burned mice challenged with Pseudomonas. Male mice (C57/BL/6) received a full thickness burn of 35% of their total body surface area. Mice received 5 IU/kg insulin i.p. or an equal volume of saline for 5 days after burn. Mice were challenged with 5 × 10 colony forming units Pseudomonas aeruginosa intraperitoneally. Serum was harvested 6 h after the bacterial challenge, and 18 serum cytokines were measured using the Bio-Plex suspension array system (Bio-Rad, Hercules, Calif). All 18 cytokines were elevated after the Pseudomonas challenge.However, mice treated with insulin showed significantly lower proinflammatory cytokine concentrations of interleukin 5, interleukin 6, and keratinocyte-derived chemokine after the Pseudomonas infection when compared with placebo-treated mice (P < 0.05). In contrast, serum concentrations of G-CSF were significantly higher in insulin-treated animals when compared with placebo (P < 0.05). We conclude, that insulin treatment selectively modulates specific cytokines in a burn wound infection model.

Original languageEnglish (US)
Pages (from-to)205-208
Number of pages4
JournalShock
Volume27
Issue number2
DOIs
StatePublished - Feb 2007

Fingerprint

Wound Infection
Insulin
Cytokines
Pseudomonas
Serum
Placebos
Pseudomonas Infections
Body Surface Area
Interleukin-5
Granulocyte Colony-Stimulating Factor
Pseudomonas aeruginosa
Immune System
Interleukin-6
Suspensions
Stem Cells
Morbidity
Mortality
Wounds and Injuries
Infection

Keywords

  • Infection
  • Inflammatory response
  • Mice
  • Pseudomonas aeruginosa
  • Sepsis

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Insulin attenuates the cytokine response in a burn wound infection model. / Przkora, Rene; Herndon, David; Finnerty, Celeste; Jeschke, Marc G.

In: Shock, Vol. 27, No. 2, 02.2007, p. 205-208.

Research output: Contribution to journalArticle

Przkora, Rene ; Herndon, David ; Finnerty, Celeste ; Jeschke, Marc G. / Insulin attenuates the cytokine response in a burn wound infection model. In: Shock. 2007 ; Vol. 27, No. 2. pp. 205-208.
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