Insulin increases mRNA abundance of the amino acid transporter SLC7A5/LAT1 via an mTORC1-dependent mechanism in skeletal muscle cells

Dillon K. Walker, Micah J. Drummond, Jared M. Dickinson, Michael S. Borack, Kristofer Jennings, Elena Volpi, Blake Rasmussen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Amino acid transporters (AATs) provide a link between amino acid availability and mammalian/mechanistic target of rapamycin complex 1 (mTORC1) activation although the direct relationship remains unclear. Previous studies in various cell types have used high insulin concentrations to determine the role of insulin on mTORC1 signaling and AAT mRNA abundance. However, this approach may limit applicability to human physiology. Therefore, we sought to determine the effect of insulin on mTORC1 signaling and whether lower insulin concentrations stimulate AAT mRNA abundance in muscle cells. We hypothesized that lower insulin concentrations would increase mRNA abundance of select AAT via an mTORC1-dependent mechanism in C2C12 myotubes. Insulin (0.5 nmol/L) significantly increased phosphorylation of the mTORC1 downstream effectors p70 ribosomal protein S6 kinase 1 (S6K1) and ribosomal protein S6 (S6). A low rapamycin dose (2.5 nmol/L) significantly reduced the insulin-(0.5 nmol/L) stimulated S6K1 and S6 phosphorylation. A high rapamycin dose (50 nmol/L) further reduced the insulin-(0.5 nmol/L) stimulated phosphorylation of S6K1 and S6. Insulin (0.5 nmol/L) increased mRNA abundance of SLC38A2/SNAT2 (P ≤ 0.043) and SLC7A5/LAT1 (P ≤ 0.021) at 240 min and SLC36A1/PAT1 (P = 0.039) at 30 min. High rapamycin prevented an increase in SLC38A2/SNAT2 (P = 0.075) and SLC36A1/PAT1 (P ≥ 0.06) mRNA abundance whereas both rapamycin doses prevented an increase in SLC7A5/LAT1 (P ≥ 0.902) mRNA abundance. We conclude that a low insulin concentration increases SLC7A5/LAT1 mRNA abundance in an mTORC1-dependent manner in skeletal muscle cells.

Original languageEnglish (US)
Article numbere00238
JournalPhysiological Reports
Volume2
Issue number3
DOIs
StatePublished - 2014

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Large Neutral Amino Acid-Transporter 1
Amino Acid Transport Systems
Muscle Cells
Skeletal Muscle
Insulin
Messenger RNA
Sirolimus
Ribosomal Protein S6 Kinases
S 6
Phosphorylation
Ribosomal Protein S6
mechanistic target of rapamycin complex 1
Skeletal Muscle Fibers

Keywords

  • C2C12 myotubes
  • PAT1
  • Rapamycin
  • SLC7A5
  • SNAT2

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology

Cite this

Insulin increases mRNA abundance of the amino acid transporter SLC7A5/LAT1 via an mTORC1-dependent mechanism in skeletal muscle cells. / Walker, Dillon K.; Drummond, Micah J.; Dickinson, Jared M.; Borack, Michael S.; Jennings, Kristofer; Volpi, Elena; Rasmussen, Blake.

In: Physiological Reports, Vol. 2, No. 3, e00238, 2014.

Research output: Contribution to journalArticle

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AU - Borack, Michael S.

AU - Jennings, Kristofer

AU - Volpi, Elena

AU - Rasmussen, Blake

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