Insulin-like growth factor-I cDNA gene transfer in vitro and in vivo

Z. Tao, David Herndon, H. Hawkins, Thomas Wood, R. Perez-Polo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Our hypothesis is that gene transfer of an IGF-I CMV-cDNA with cholesterol containing cationic liposomes is an efficient tool for transient transfection of growth factors in vitro and in vivo. In vitro, we transiently cotransfected IGF-I cDNA with a CMV construct and a Lac Z β-galactosidase cDNA/CMV construct using cholesterol containing cationic liposomes and measured β-galactosidase and IGF-I mRNA and protein. In vivo, we subcutaneously injected 3-month-old male Sprague-Dawley rats with IGF-I cDNA and β-galactosidase cDNA into rat skin. After IGF-I and β-galactosidase were cotransfected into PCI2 cells, Northern blot analysis showed that the peak time of IGF-I expression was 2 days for mRNA and 5 days for protein. In vivo, a cDNA/liposome ratio of 1:2 was most effective. IGF-I protein expression in IGF-I-transfected skin resulted in significant transfection from day 5 to day 7. In situ determination of β-galactosidase activity confirmed that transfections resulted in a restricted expression area.

Original languageEnglish (US)
Pages (from-to)41-55
Number of pages15
JournalBiochemical Genetics
Volume38
Issue number1-2
StatePublished - 2000

Fingerprint

Gene transfer
gene transfer
insulin-like growth factor I
Insulin-Like Growth Factor I
Galactosidases
galactosidases
Complementary DNA
protein
skin
Genes
transfection
Liposomes
Transfection
Rats
Skin
Cholesterol
cholesterol
Messenger RNA
Proteins
In Vitro Techniques

Keywords

  • Gene transfer
  • IGF-I
  • Liposome
  • Rat
  • Wound healing

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Tao, Z., Herndon, D., Hawkins, H., Wood, T., & Perez-Polo, R. (2000). Insulin-like growth factor-I cDNA gene transfer in vitro and in vivo. Biochemical Genetics, 38(1-2), 41-55.

Insulin-like growth factor-I cDNA gene transfer in vitro and in vivo. / Tao, Z.; Herndon, David; Hawkins, H.; Wood, Thomas; Perez-Polo, R.

In: Biochemical Genetics, Vol. 38, No. 1-2, 2000, p. 41-55.

Research output: Contribution to journalArticle

Tao, Z, Herndon, D, Hawkins, H, Wood, T & Perez-Polo, R 2000, 'Insulin-like growth factor-I cDNA gene transfer in vitro and in vivo', Biochemical Genetics, vol. 38, no. 1-2, pp. 41-55.
Tao Z, Herndon D, Hawkins H, Wood T, Perez-Polo R. Insulin-like growth factor-I cDNA gene transfer in vitro and in vivo. Biochemical Genetics. 2000;38(1-2):41-55.
Tao, Z. ; Herndon, David ; Hawkins, H. ; Wood, Thomas ; Perez-Polo, R. / Insulin-like growth factor-I cDNA gene transfer in vitro and in vivo. In: Biochemical Genetics. 2000 ; Vol. 38, No. 1-2. pp. 41-55.
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