Insulin-like growth factoristimulates elastin synthesis by bovine pulmonary arterial smooth muscle cells

D. B. Badesch, Phillip Lee, W. C. Parks, K. R. Stenmark

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Insulin-like growth factor I stimulates mitogenesis in smooth muscle cells, and upregulates elastin synthesis in embryonic aortic tissue. Increased smooth muscle elastin synthesis may play an important role in vascular remodeling in chronic pulmonary hypertension. Therefore, we studied the effect of IGF-I on elastin and total protein synthesis by pulmonary arterial smooth muscle cells in vitro. Tropoelastin synthesis was measured by enzyme immunoassay, and total protein synthesis was measured by [3H]-leucine incorporation. In addition, the steady-state levels of tropoelastin mRNA were determined by slot blot hybridization. Incubation of confluent cultures with various concentrations of IGF-I resulted in a dose-dependent stimulation of elastin synthesis, with a 2.4-fold increase over control levels at 1000 ng/ml of IGF. The increase in elastin synthesis was reflected by a stimulation of the steady-state levels of tropoelastin mRNA. We conclude that IGF-I has potent elastogenic effects on vascular smooth muscle cells, and speculate that it may contribute to vascular wall remodeling in chronic hypertension.

Original languageEnglish (US)
Pages (from-to)382-387
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume160
Issue number1
DOIs
StatePublished - Apr 14 1989
Externally publishedYes

Fingerprint

Elastin
Tropoelastin
Smooth Muscle Myocytes
Muscle
Insulin-Like Growth Factor I
Cells
Insulin
Lung
Growth
Messenger RNA
Immunoenzyme Techniques
Vascular Smooth Muscle
Pulmonary Hypertension
Leucine
Smooth Muscle
Proteins
Level control
Up-Regulation
Hypertension
Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Insulin-like growth factoristimulates elastin synthesis by bovine pulmonary arterial smooth muscle cells. / Badesch, D. B.; Lee, Phillip; Parks, W. C.; Stenmark, K. R.

In: Biochemical and Biophysical Research Communications, Vol. 160, No. 1, 14.04.1989, p. 382-387.

Research output: Contribution to journalArticle

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