Insulin-like growth factors enhance phagocytosis by human neutrophils in vitro

Gui Fang Jin, Yan Shi Guo, Chuck Ball, Clifford W. Houston

    Research output: Contribution to journalArticle

    14 Scopus citations

    Abstract

    Polymorphonuclear neutrophils (PMNs) were isolated from human blood, and PMN phagocytosis was assessed by measuring the chemiluminescence (CL) response in the presence of ZAP (opsonized zymosin particles containing luminol). The administration of 6.5 nM of insulin-like growth factor I (IGF-I), des(1-3)-IGF-I, IGF-II or insulin to PMNs for 20 min resulted in significant increases of the CL response for all test preparations. Des(1-3)-IGF-I, a truncated IGF-I with low affinity binding to IGF binding proteins (IGFBPs), was the most potent CL stimulator. The CL production evoked by 6.5 nM of des(1-3)-IGF-I was inhibited significantly by both 0.25 and 1.0 mM of EGTA (Ca2+ chelator), or 10 μM nifedipine (Ca2+ channel inhibitor), pertussis toxin (0.05 and 1.0 μg/ml) or cholera toxin (5 μg/ml). These results suggest that IGF-I and its homologues are potent stimulators of phagocytosis and that this action is modulated by IGFBP, and may require extracellular Ca2+ and/or IGF-I receptor G-protein coupling.

    Original languageEnglish (US)
    Pages (from-to)125-131
    Number of pages7
    JournalRegulatory Peptides
    Volume49
    Issue number2
    DOIs
    StatePublished - Dec 10 1993

    Keywords

    • Chemiluminescence
    • EGTA
    • G-protein
    • Insulin-like growth factor
    • Nifedipine
    • Phagocytosis

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology
    • Endocrinology
    • Clinical Biochemistry
    • Cellular and Molecular Neuroscience

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