Insulin regulation of insulin-like growth factor-binding protein production in cultured HepG2 cells

C. A. Conover, Phillip Lee

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

A human hepatoma cell line, HepG2, secretes a discrete insulin-like growth factor-binding protein (IGFBP-1) into serum-free medium, which is identical to the 25K mol wt BP in amniotic fluid and plasma. IGFBP-1 levels in vivo have been shown to be inversely correlated with circulating insulin concentrations. This study investigated the direct effects of insulin on IGFBP-1 production in vitro. Addition of insulin to HepG2 cultures induced a rapid dose-dependent decrease in IGFBP-1 synthesis and secretion independent of the glucose concentration in the medium. As assessed by ligand binding and specific RIA, levels of IGFBP-1 were 20-50% of control levels in 18-h conditioned medium from insulin-treated cells. Monoclonal antibody studies indicated that the suppressive effect of insulin on IGFBP-1 synthesis was mediated through specific interaction with the insulin receptor. Therefore, HepG2 cells respond to insulin by altering the synthesis and secretion of IGFBP-1 in a manner that mimics many of the changes in plasma IGFBP-1 levels observed in vivo and provide an in vitro model for studies of IGFBP-1 biosynthesis.

Original languageEnglish (US)
Pages (from-to)1062-1067
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume70
Issue number4
StatePublished - Apr 1990
Externally publishedYes

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Insulin-Like Growth Factor Binding Protein 1
Insulin-Like Growth Factor Binding Proteins
Hep G2 Cells
Cultured Cells
Insulin
Cells
Plasmas
Biosynthesis
Level control
Insulin Receptor
Serum-Free Culture Media
Conditioned Culture Medium
Amniotic Fluid
Hepatocellular Carcinoma
Monoclonal Antibodies
Ligands
Glucose

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Insulin regulation of insulin-like growth factor-binding protein production in cultured HepG2 cells. / Conover, C. A.; Lee, Phillip.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 70, No. 4, 04.1990, p. 1062-1067.

Research output: Contribution to journalArticle

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