Insulin sensitivity is related to fat oxidation and protein kinase C activity in children with acute burn injury

Melanie G. Cree, Jennifer J. Zwetsloot, David Herndon, Bradley R. Newcomer, Ricki Y. Fram, Carlos Angel, Justin M. Green, Gerald L. Dohm, Dayoung Sun, Asle Aarsland, Robert R. Wolfe

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Impaired fatty acid oxidation occurs with type 2 diabetes and is associated with accumulations of intracellular lipids, which may increase diacylglycerol (DAG), stimulate protein kinase C activity, and inactivate insulin signaling. Glucose and fat metabolism are altered in burn patients, but have never been related to intracellular lipids or insulin signaling. Thirty children sustaining > 40% total body surface area burns were studied acutely with glucose and palmitate tracer infusions and a hyper-insulinemic euglycemic clamp. Muscle triglyceride,DAG, fatty acyl CoA, and insulin signaling were measured. Liver and muscle triglyceride levels were measured with magnetic resonance spectroscopy. Muscle samples from healthy children were controls for DAG concentrations. Insulin sensitivity was reduced and correlated with whole body palmitate β-oxidation (P= .004). Muscle insulin signaling was not stimulated by hyper-insulinemia. Tissue triglyceride concentrations and activated protein kinase C-β were elevated, whereas the concentration of DAG was similar to the controls. Free fatty acid profiles of muscle triglyceride did not match DAG. Insulin resistance following burn injury is accompanied by decreased insulin signaling and increased protein kinase C-β activation. The best metabolic predictor of insulin resistance in burned patients was palmitate oxidation.

Original languageEnglish (US)
Pages (from-to)585-594
Number of pages10
JournalJournal of Burn Care and Research
Volume29
Issue number4
DOIs
StatePublished - Jul 2008

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Diglycerides
Protein Kinase C
Insulin Resistance
Fats
Insulin
Palmitates
Triglycerides
Muscles
Wounds and Injuries
Lipids
Glucose
Acyl Coenzyme A
Glucose Clamp Technique
Body Surface Area
Burns
Nonesterified Fatty Acids
Type 2 Diabetes Mellitus
Magnetic Resonance Spectroscopy
Fatty Acids
Liver

ASJC Scopus subject areas

  • Emergency Medicine
  • Rehabilitation
  • Surgery

Cite this

Cree, M. G., Zwetsloot, J. J., Herndon, D., Newcomer, B. R., Fram, R. Y., Angel, C., ... Wolfe, R. R. (2008). Insulin sensitivity is related to fat oxidation and protein kinase C activity in children with acute burn injury. Journal of Burn Care and Research, 29(4), 585-594. https://doi.org/10.1097/BCR.0b013e31817db88f

Insulin sensitivity is related to fat oxidation and protein kinase C activity in children with acute burn injury. / Cree, Melanie G.; Zwetsloot, Jennifer J.; Herndon, David; Newcomer, Bradley R.; Fram, Ricki Y.; Angel, Carlos; Green, Justin M.; Dohm, Gerald L.; Sun, Dayoung; Aarsland, Asle; Wolfe, Robert R.

In: Journal of Burn Care and Research, Vol. 29, No. 4, 07.2008, p. 585-594.

Research output: Contribution to journalArticle

Cree, MG, Zwetsloot, JJ, Herndon, D, Newcomer, BR, Fram, RY, Angel, C, Green, JM, Dohm, GL, Sun, D, Aarsland, A & Wolfe, RR 2008, 'Insulin sensitivity is related to fat oxidation and protein kinase C activity in children with acute burn injury', Journal of Burn Care and Research, vol. 29, no. 4, pp. 585-594. https://doi.org/10.1097/BCR.0b013e31817db88f
Cree, Melanie G. ; Zwetsloot, Jennifer J. ; Herndon, David ; Newcomer, Bradley R. ; Fram, Ricki Y. ; Angel, Carlos ; Green, Justin M. ; Dohm, Gerald L. ; Sun, Dayoung ; Aarsland, Asle ; Wolfe, Robert R. / Insulin sensitivity is related to fat oxidation and protein kinase C activity in children with acute burn injury. In: Journal of Burn Care and Research. 2008 ; Vol. 29, No. 4. pp. 585-594.
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