Insulinlike growth factor 1 (IGF-1) reduces gut atrophy and bacterial translocation after severe burn injury

K. F. Huang, D. H. Chung, David Herndon

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Bacterial translocation after severe burns is associated with gut mucosal atrophy and increased mucosal permeability. Insulinlike growth factor 1 (IGF- 1) levels are low after trauma and do not respond to growth hormone treatment. Since IGF-1 receptors have been demonstrated in gut mucosa, we proposed that treatment with IGF-1 would reduce mucosal atrophy and bacterial translocation. Rats received 50% total body surface area full-thickness burn or sham burn. They were treated with a continuous, subcutaneous infusion of either IGF-1 (≃3 mg/kg per day) or placebo (0.01 mol of acetate) for up to 5 days after receiving the burn. The mesenteric lymph node and liver were cultured for gram-negative bacteria. The small intestinal mucosa was scraped, weighed, and analyzed for DNA and protein content. Treatment with IGF-1 improved body weight, spleen weight, and gut mucosal weight. It stimulated mucosal DNA and protein content and reduced the incidence of bacterial translocation to the mesenteric lymph node from 89% to 30%. Insulinlike growth factor may reduce gut barrier failure by decreasing mucosal atrophy and subsequent barrier failure. In addition to its general anabolic effects, recombinant human IGF-1 may improve gut mucosal function and reduce infectious morbidity in severely traumatized or septic patients by reducing gut atrophy and reducing bacterial translocation.

Original languageEnglish (US)
Pages (from-to)47-54
Number of pages8
JournalArchives of Surgery
Volume128
Issue number1
StatePublished - 1993

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Bacterial Translocation
Atrophy
Intercellular Signaling Peptides and Proteins
Wounds and Injuries
Lymph Nodes
Anabolic Agents
Weights and Measures
Subcutaneous Infusions
Growth Factor Receptors
Body Surface Area
DNA
Intestinal Mucosa
Gram-Negative Bacteria
Burns
Growth Hormone
Permeability
Mucous Membrane
Proteins
Acetates
Therapeutics

ASJC Scopus subject areas

  • Surgery

Cite this

Insulinlike growth factor 1 (IGF-1) reduces gut atrophy and bacterial translocation after severe burn injury. / Huang, K. F.; Chung, D. H.; Herndon, David.

In: Archives of Surgery, Vol. 128, No. 1, 1993, p. 47-54.

Research output: Contribution to journalArticle

Huang, K. F. ; Chung, D. H. ; Herndon, David. / Insulinlike growth factor 1 (IGF-1) reduces gut atrophy and bacterial translocation after severe burn injury. In: Archives of Surgery. 1993 ; Vol. 128, No. 1. pp. 47-54.
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