Insulinlike growth factor I in combination with insulinlike growth factor binding protein 3 affects the hepatic acute phase response and hepatic morphology in thermally injured rats

Marc G. Jeschke, David Herndon, Robert E. Barrow

Research output: Contribution to journalArticle

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Abstract

Objective: To modulate the hepatic acute phase response after a thermal injury by the administration of insulinlike growth factor I (IGF-1) in combination with its principal binding protein 3 (IGFBP-3). Summary Background Data: The hepatic acute phase response is a cascade of events initiated to restore homeostasis after trauma; however, a prolonged response contributes to multiorgan failure, hypermetabolism, complications, and death. Although IGF-1 has been shown to improve cell recovery and play a major role in liver regeneration, its effect on the hepatic acute phase response is not known. Methods: Sprague-Dawley rats (56 males) received a 60% total body surface area third-degree scald bum and were randomly divided to receive either rhIGF-I/BP-3 (10 mg/kg/day given subcutaneously) or saline (control). Rats were killed on postburn days 1,2, 5, and 7 and serum glucose, electrolytes, acute phase reactant proteins, tumor necrosis factor α, interleukin 1α, interleukin 6, and rat and human serum IGF-1 and IGFBP-3 were measured. Hepatic protein concentrations, hepatocyte proliferation, and hepatocyte apoptosis were determined. Results: No hypoglycemia or electrolyte imbalance could be shown in rats receiving the growth factor complex compared with saline. rhIGF-I/BP-3 increased serum protein on postburn days 2 and 7, albumin on days 5 and 7, and transferrin on days 1, 5, and 7, and decreased haptoglobin and α1-acid glycoprotein on postburn days 5 and 7 compared with controls. IGF-I/BP-3 had no effect on type II acute phase proteins. Rats receiving IGF-I/BP-3 had lower serum levels of interleukin 1β and tumor necrosis factor α on the first day after burn compared with controls, whereas serum levels of interleukin 6 did not change, rhIGF-I/BP-3 significantly increased total liver protein content on postburn days 1, 2, 5, and 7 compared with controls. IGF-I/BP-3 increased hepatocyte proliferation and decreased hepatocyte apoptosis versus controls. Conclusion: In combination with its principal binding protein, rhIGF-I decreases the proinflammatory cytokines interleukin 1β and tumor necrosis factor α, followed by a decrease in type I acute phase proteins. IGF-I/BP-3 had no effect on interleukin 6 and type II acute phase proteins. Decreases in acute phase protein and proinflammatory cytokine synthesis were associated with increases in constitutive hepatic proteins, total liver protein content, and hepatocyte proliferation. IGF-I/BP-3 attenuates the hypermetabolic response after thermal injury and may improve the clinical outcome.

Original languageEnglish (US)
Pages (from-to)408-416
Number of pages9
JournalAnnals of Surgery
Volume231
Issue number3
DOIs
StatePublished - Mar 2000

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Acute-Phase Reaction
Insulin-Like Growth Factor I
Fibrinogen
Acute-Phase Proteins
Intercellular Signaling Peptides and Proteins
Carrier Proteins
Liver
Hepatocytes
Interleukin-1
Interleukin-6
Insulin-Like Growth Factor Binding Protein 3
Tumor Necrosis Factor-alpha
Serum
Electrolytes
Wounds and Injuries
Proteins
Hot Temperature
Apoptosis
Cytokines
Liver Regeneration

ASJC Scopus subject areas

  • Surgery

Cite this

Insulinlike growth factor I in combination with insulinlike growth factor binding protein 3 affects the hepatic acute phase response and hepatic morphology in thermally injured rats. / Jeschke, Marc G.; Herndon, David; Barrow, Robert E.

In: Annals of Surgery, Vol. 231, No. 3, 03.2000, p. 408-416.

Research output: Contribution to journalArticle

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abstract = "Objective: To modulate the hepatic acute phase response after a thermal injury by the administration of insulinlike growth factor I (IGF-1) in combination with its principal binding protein 3 (IGFBP-3). Summary Background Data: The hepatic acute phase response is a cascade of events initiated to restore homeostasis after trauma; however, a prolonged response contributes to multiorgan failure, hypermetabolism, complications, and death. Although IGF-1 has been shown to improve cell recovery and play a major role in liver regeneration, its effect on the hepatic acute phase response is not known. Methods: Sprague-Dawley rats (56 males) received a 60{\%} total body surface area third-degree scald bum and were randomly divided to receive either rhIGF-I/BP-3 (10 mg/kg/day given subcutaneously) or saline (control). Rats were killed on postburn days 1,2, 5, and 7 and serum glucose, electrolytes, acute phase reactant proteins, tumor necrosis factor α, interleukin 1α, interleukin 6, and rat and human serum IGF-1 and IGFBP-3 were measured. Hepatic protein concentrations, hepatocyte proliferation, and hepatocyte apoptosis were determined. Results: No hypoglycemia or electrolyte imbalance could be shown in rats receiving the growth factor complex compared with saline. rhIGF-I/BP-3 increased serum protein on postburn days 2 and 7, albumin on days 5 and 7, and transferrin on days 1, 5, and 7, and decreased haptoglobin and α1-acid glycoprotein on postburn days 5 and 7 compared with controls. IGF-I/BP-3 had no effect on type II acute phase proteins. Rats receiving IGF-I/BP-3 had lower serum levels of interleukin 1β and tumor necrosis factor α on the first day after burn compared with controls, whereas serum levels of interleukin 6 did not change, rhIGF-I/BP-3 significantly increased total liver protein content on postburn days 1, 2, 5, and 7 compared with controls. IGF-I/BP-3 increased hepatocyte proliferation and decreased hepatocyte apoptosis versus controls. Conclusion: In combination with its principal binding protein, rhIGF-I decreases the proinflammatory cytokines interleukin 1β and tumor necrosis factor α, followed by a decrease in type I acute phase proteins. IGF-I/BP-3 had no effect on interleukin 6 and type II acute phase proteins. Decreases in acute phase protein and proinflammatory cytokine synthesis were associated with increases in constitutive hepatic proteins, total liver protein content, and hepatocyte proliferation. IGF-I/BP-3 attenuates the hypermetabolic response after thermal injury and may improve the clinical outcome.",
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T1 - Insulinlike growth factor I in combination with insulinlike growth factor binding protein 3 affects the hepatic acute phase response and hepatic morphology in thermally injured rats

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N2 - Objective: To modulate the hepatic acute phase response after a thermal injury by the administration of insulinlike growth factor I (IGF-1) in combination with its principal binding protein 3 (IGFBP-3). Summary Background Data: The hepatic acute phase response is a cascade of events initiated to restore homeostasis after trauma; however, a prolonged response contributes to multiorgan failure, hypermetabolism, complications, and death. Although IGF-1 has been shown to improve cell recovery and play a major role in liver regeneration, its effect on the hepatic acute phase response is not known. Methods: Sprague-Dawley rats (56 males) received a 60% total body surface area third-degree scald bum and were randomly divided to receive either rhIGF-I/BP-3 (10 mg/kg/day given subcutaneously) or saline (control). Rats were killed on postburn days 1,2, 5, and 7 and serum glucose, electrolytes, acute phase reactant proteins, tumor necrosis factor α, interleukin 1α, interleukin 6, and rat and human serum IGF-1 and IGFBP-3 were measured. Hepatic protein concentrations, hepatocyte proliferation, and hepatocyte apoptosis were determined. Results: No hypoglycemia or electrolyte imbalance could be shown in rats receiving the growth factor complex compared with saline. rhIGF-I/BP-3 increased serum protein on postburn days 2 and 7, albumin on days 5 and 7, and transferrin on days 1, 5, and 7, and decreased haptoglobin and α1-acid glycoprotein on postburn days 5 and 7 compared with controls. IGF-I/BP-3 had no effect on type II acute phase proteins. Rats receiving IGF-I/BP-3 had lower serum levels of interleukin 1β and tumor necrosis factor α on the first day after burn compared with controls, whereas serum levels of interleukin 6 did not change, rhIGF-I/BP-3 significantly increased total liver protein content on postburn days 1, 2, 5, and 7 compared with controls. IGF-I/BP-3 increased hepatocyte proliferation and decreased hepatocyte apoptosis versus controls. Conclusion: In combination with its principal binding protein, rhIGF-I decreases the proinflammatory cytokines interleukin 1β and tumor necrosis factor α, followed by a decrease in type I acute phase proteins. IGF-I/BP-3 had no effect on interleukin 6 and type II acute phase proteins. Decreases in acute phase protein and proinflammatory cytokine synthesis were associated with increases in constitutive hepatic proteins, total liver protein content, and hepatocyte proliferation. IGF-I/BP-3 attenuates the hypermetabolic response after thermal injury and may improve the clinical outcome.

AB - Objective: To modulate the hepatic acute phase response after a thermal injury by the administration of insulinlike growth factor I (IGF-1) in combination with its principal binding protein 3 (IGFBP-3). Summary Background Data: The hepatic acute phase response is a cascade of events initiated to restore homeostasis after trauma; however, a prolonged response contributes to multiorgan failure, hypermetabolism, complications, and death. Although IGF-1 has been shown to improve cell recovery and play a major role in liver regeneration, its effect on the hepatic acute phase response is not known. Methods: Sprague-Dawley rats (56 males) received a 60% total body surface area third-degree scald bum and were randomly divided to receive either rhIGF-I/BP-3 (10 mg/kg/day given subcutaneously) or saline (control). Rats were killed on postburn days 1,2, 5, and 7 and serum glucose, electrolytes, acute phase reactant proteins, tumor necrosis factor α, interleukin 1α, interleukin 6, and rat and human serum IGF-1 and IGFBP-3 were measured. Hepatic protein concentrations, hepatocyte proliferation, and hepatocyte apoptosis were determined. Results: No hypoglycemia or electrolyte imbalance could be shown in rats receiving the growth factor complex compared with saline. rhIGF-I/BP-3 increased serum protein on postburn days 2 and 7, albumin on days 5 and 7, and transferrin on days 1, 5, and 7, and decreased haptoglobin and α1-acid glycoprotein on postburn days 5 and 7 compared with controls. IGF-I/BP-3 had no effect on type II acute phase proteins. Rats receiving IGF-I/BP-3 had lower serum levels of interleukin 1β and tumor necrosis factor α on the first day after burn compared with controls, whereas serum levels of interleukin 6 did not change, rhIGF-I/BP-3 significantly increased total liver protein content on postburn days 1, 2, 5, and 7 compared with controls. IGF-I/BP-3 increased hepatocyte proliferation and decreased hepatocyte apoptosis versus controls. Conclusion: In combination with its principal binding protein, rhIGF-I decreases the proinflammatory cytokines interleukin 1β and tumor necrosis factor α, followed by a decrease in type I acute phase proteins. IGF-I/BP-3 had no effect on interleukin 6 and type II acute phase proteins. Decreases in acute phase protein and proinflammatory cytokine synthesis were associated with increases in constitutive hepatic proteins, total liver protein content, and hepatocyte proliferation. IGF-I/BP-3 attenuates the hypermetabolic response after thermal injury and may improve the clinical outcome.

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