Integrative multi-omics deciphers the spatial characteristics of host-gut microbiota interactions in Crohn's disease

Xiang Gao, Ruicong Sun, Na Jiao, Xiao Liang, Gengfeng Li, Han Gao, Xiaohan Wu, Muqing Yang, Chunqiu Chen, Xiaomin Sun, Liang Chen, Wei Wu, Yingzi Cong, Ruixin Zhu, Tiannan Guo, Zhanju Liu

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Dysregulated host-microbial interactions play critical roles in initiation and perpetuation of gut inflammation in Crohn's disease (CD). However, the spatial distribution and interaction network across the intestine and its accessory tissues are still elusive. Here, we profile the host proteins and tissue microbes in 540 samples from the intestinal mucosa, submucosa-muscularis-serosa, mesenteric adipose tissues, mesentery, and mesenteric lymph nodes of 30 CD patients and spatially decipher the host-microbial interactions. We observe aberrant antimicrobial immunity and metabolic processes across multi-tissues during CD and determine bacterial transmission along with altered microbial communities and ecological patterns. Moreover, we identify several candidate interaction pairs between host proteins and microbes associated with perpetuation of gut inflammation and bacterial transmigration across multi-tissues in CD. Signature alterations in host proteins (e.g., SAA2 and GOLM1) and microbes (e.g., Alistipes and Streptococcus) are further imprinted in serum and fecal samples as potential diagnostic biomarkers, thus providing a rationale for precision diagnosis.

Original languageEnglish (US)
Article number101050
JournalCell Reports Medicine
Volume4
Issue number6
DOIs
StatePublished - Jun 20 2023

Keywords

  • Crohn disease
  • interaction pattern
  • microbiome
  • proteome
  • spatial characteristics

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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