Interaction between bactrachotoxin and yohimbine

Li-Yen Huang, G. Ehrenstein, W. A. Catterall

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The neurotoxins, batrachotoxin and veratridine, are specific activators of sodium channels and cause an increase in the rate of 22Na uptake neuroblastoma cells. Yohimbine, an indolalkylamine alkaloid, inhibits this batrachotoxin-induced 22Na uptake. The dose-response curve of yohimbine suggests that the inhibitor acts reversibly on a single class of binding sites with dissociation constant of 3-4 x 10-5M. The dissociation constant is not affected by depolarization from -41 to 0 mV. Kinetic and equilibrium experiments indicate that yohimbine is a competitive inhibitor of the action of batrachotoxin. These results support the conclusion that yohimbine inhibits the sodium flux by acting on the channel gating mechanism rather than by occluding the channels.

Original languageEnglish (US)
Pages (from-to)219-231
Number of pages13
JournalBiophysical Journal
Volume23
Issue number2
StatePublished - 1978
Externally publishedYes

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Batrachotoxins
Yohimbine
Sodium Channel Agonists
Veratridine
Neurotoxins
Neuroblastoma
Alkaloids
Sodium
Binding Sites

ASJC Scopus subject areas

  • Biophysics

Cite this

Huang, L-Y., Ehrenstein, G., & Catterall, W. A. (1978). Interaction between bactrachotoxin and yohimbine. Biophysical Journal, 23(2), 219-231.

Interaction between bactrachotoxin and yohimbine. / Huang, Li-Yen; Ehrenstein, G.; Catterall, W. A.

In: Biophysical Journal, Vol. 23, No. 2, 1978, p. 219-231.

Research output: Contribution to journalArticle

Huang, L-Y, Ehrenstein, G & Catterall, WA 1978, 'Interaction between bactrachotoxin and yohimbine', Biophysical Journal, vol. 23, no. 2, pp. 219-231.
Huang L-Y, Ehrenstein G, Catterall WA. Interaction between bactrachotoxin and yohimbine. Biophysical Journal. 1978;23(2):219-231.
Huang, Li-Yen ; Ehrenstein, G. ; Catterall, W. A. / Interaction between bactrachotoxin and yohimbine. In: Biophysical Journal. 1978 ; Vol. 23, No. 2. pp. 219-231.
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