Interaction of 16β-hydroxydehydroepiandrosterone with renal mineralocorticoid receptors

John R. Higgins; Gerhard Wambach; David C. Kem; Celso Gomez-Sanchez; O. Bryan Holland; Norman M. Kaplan

Interaction of 16β-hydroxydehydroepiandrosterone with renal mineralocorticoid receptors

John R. Higgins, Gerhard Wambach, David C. Kem, Celso Gomez-Sanchez, O. Bryan Holland, Norman M. Kaplan

Research output: Contribution to journal › Article › peer-review

Abstract

A previously unidentified mineralocorticoid, 16β-hydroxydehydroepiandrosterone (16β OH-DHEA) has recently been isolated from urine extracts of patients with low-renin hypertension and reported to possess one-fortieth the mineralocorticoid potency of aldosterone by bioassay. However, using a radioreceptor assay, we demonstrate the affinity of 16β-OH-DHEA for renal ³H-aldosterone receptors to be only 0.011 per cent that of unlabeled aldosterone. 16β-OH-DHEA therefore would not be expected to possess significant mineralocorticoid properties unless its mechanism of action involves binding to a unique class of renal receptors. Until these discrepancies between bioassayable mineralocorticoid activity and affinity for mineralocorticoid receptors can be resolved, the role of 16β-OH-DHEA in the pathogenesis of low-renin hypertension remains speculative.

Original language	English (US)
Pages (from-to)	250-256
Number of pages	7
Journal	The Journal of Laboratory and Clinical Medicine
Volume	89
Issue number	2
State	Published - Feb 1977
Externally published	Yes

ASJC Scopus subject areas

Pathology and Forensic Medicine

Cite this

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title = "Interaction of 16β-hydroxydehydroepiandrosterone with renal mineralocorticoid receptors",

abstract = "A previously unidentified mineralocorticoid, 16β-hydroxydehydroepiandrosterone (16β OH-DHEA) has recently been isolated from urine extracts of patients with low-renin hypertension and reported to possess one-fortieth the mineralocorticoid potency of aldosterone by bioassay. However, using a radioreceptor assay, we demonstrate the affinity of 16β-OH-DHEA for renal 3H-aldosterone receptors to be only 0.011 per cent that of unlabeled aldosterone. 16β-OH-DHEA therefore would not be expected to possess significant mineralocorticoid properties unless its mechanism of action involves binding to a unique class of renal receptors. Until these discrepancies between bioassayable mineralocorticoid activity and affinity for mineralocorticoid receptors can be resolved, the role of 16β-OH-DHEA in the pathogenesis of low-renin hypertension remains speculative.",

author = "Higgins, {John R.} and Gerhard Wambach and Kem, {David C.} and Celso Gomez-Sanchez and Holland, {O. Bryan} and Kaplan, {Norman M.}",

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TY - JOUR

T1 - Interaction of 16β-hydroxydehydroepiandrosterone with renal mineralocorticoid receptors

AU - Higgins, John R.

AU - Wambach, Gerhard

AU - Kem, David C.

AU - Gomez-Sanchez, Celso

AU - Holland, O. Bryan

AU - Kaplan, Norman M.

PY - 1977/2

Y1 - 1977/2

N2 - A previously unidentified mineralocorticoid, 16β-hydroxydehydroepiandrosterone (16β OH-DHEA) has recently been isolated from urine extracts of patients with low-renin hypertension and reported to possess one-fortieth the mineralocorticoid potency of aldosterone by bioassay. However, using a radioreceptor assay, we demonstrate the affinity of 16β-OH-DHEA for renal 3H-aldosterone receptors to be only 0.011 per cent that of unlabeled aldosterone. 16β-OH-DHEA therefore would not be expected to possess significant mineralocorticoid properties unless its mechanism of action involves binding to a unique class of renal receptors. Until these discrepancies between bioassayable mineralocorticoid activity and affinity for mineralocorticoid receptors can be resolved, the role of 16β-OH-DHEA in the pathogenesis of low-renin hypertension remains speculative.

AB - A previously unidentified mineralocorticoid, 16β-hydroxydehydroepiandrosterone (16β OH-DHEA) has recently been isolated from urine extracts of patients with low-renin hypertension and reported to possess one-fortieth the mineralocorticoid potency of aldosterone by bioassay. However, using a radioreceptor assay, we demonstrate the affinity of 16β-OH-DHEA for renal 3H-aldosterone receptors to be only 0.011 per cent that of unlabeled aldosterone. 16β-OH-DHEA therefore would not be expected to possess significant mineralocorticoid properties unless its mechanism of action involves binding to a unique class of renal receptors. Until these discrepancies between bioassayable mineralocorticoid activity and affinity for mineralocorticoid receptors can be resolved, the role of 16β-OH-DHEA in the pathogenesis of low-renin hypertension remains speculative.

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Interaction of 16β-hydroxydehydroepiandrosterone with renal mineralocorticoid receptors

Abstract

ASJC Scopus subject areas

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