Interaction of 16β-hydroxydehydroepiandrosterone with renal mineralocorticoid receptors

John R. Higgins, Gerhard Wambach, David C. Kem, Celso Gomez-Sanchez, O. Bryan Holland, Norman M. Kaplan

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Abstract

A previously unidentified mineralocorticoid, 16β-hydroxydehydroepiandrosterone (16β OH-DHEA) has recently been isolated from urine extracts of patients with low-renin hypertension and reported to possess one-fortieth the mineralocorticoid potency of aldosterone by bioassay. However, using a radioreceptor assay, we demonstrate the affinity of 16β-OH-DHEA for renal 3H-aldosterone receptors to be only 0.011 per cent that of unlabeled aldosterone. 16β-OH-DHEA therefore would not be expected to possess significant mineralocorticoid properties unless its mechanism of action involves binding to a unique class of renal receptors. Until these discrepancies between bioassayable mineralocorticoid activity and affinity for mineralocorticoid receptors can be resolved, the role of 16β-OH-DHEA in the pathogenesis of low-renin hypertension remains speculative.

Original languageEnglish (US)
Pages (from-to)250-256
Number of pages7
JournalThe Journal of Laboratory and Clinical Medicine
Volume89
Issue number2
StatePublished - 1977

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ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Higgins, J. R., Wambach, G., Kem, D. C., Gomez-Sanchez, C., Holland, O. B., & Kaplan, N. M. (1977). Interaction of 16β-hydroxydehydroepiandrosterone with renal mineralocorticoid receptors. The Journal of Laboratory and Clinical Medicine, 89(2), 250-256.