Interaction of 16β-hydroxydehydroepiandrosterone with renal mineralocorticoid receptors

John R. Higgins, Gerhard Wambach, David C. Kem, Celso Gomez-Sanchez, O. Bryan Holland, Norman M. Kaplan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A previously unidentified mineralocorticoid, 16β-hydroxydehydroepiandrosterone (16β OH-DHEA) has recently been isolated from urine extracts of patients with low-renin hypertension and reported to possess one-fortieth the mineralocorticoid potency of aldosterone by bioassay. However, using a radioreceptor assay, we demonstrate the affinity of 16β-OH-DHEA for renal 3H-aldosterone receptors to be only 0.011 per cent that of unlabeled aldosterone. 16β-OH-DHEA therefore would not be expected to possess significant mineralocorticoid properties unless its mechanism of action involves binding to a unique class of renal receptors. Until these discrepancies between bioassayable mineralocorticoid activity and affinity for mineralocorticoid receptors can be resolved, the role of 16β-OH-DHEA in the pathogenesis of low-renin hypertension remains speculative.

Original languageEnglish (US)
Pages (from-to)250-256
Number of pages7
JournalThe Journal of Laboratory and Clinical Medicine
Volume89
Issue number2
StatePublished - 1977

Fingerprint

Mineralocorticoid Receptors
Mineralocorticoids
Dehydroepiandrosterone
Kidney
Aldosterone
Renin
Hypertension
Radioligand Assay
Bioassay
Biological Assay
Assays
Urine
16-hydroxydehydroepiandrosterone
hydroxide ion

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Higgins, J. R., Wambach, G., Kem, D. C., Gomez-Sanchez, C., Holland, O. B., & Kaplan, N. M. (1977). Interaction of 16β-hydroxydehydroepiandrosterone with renal mineralocorticoid receptors. The Journal of Laboratory and Clinical Medicine, 89(2), 250-256.

Interaction of 16β-hydroxydehydroepiandrosterone with renal mineralocorticoid receptors. / Higgins, John R.; Wambach, Gerhard; Kem, David C.; Gomez-Sanchez, Celso; Holland, O. Bryan; Kaplan, Norman M.

In: The Journal of Laboratory and Clinical Medicine, Vol. 89, No. 2, 1977, p. 250-256.

Research output: Contribution to journalArticle

Higgins, JR, Wambach, G, Kem, DC, Gomez-Sanchez, C, Holland, OB & Kaplan, NM 1977, 'Interaction of 16β-hydroxydehydroepiandrosterone with renal mineralocorticoid receptors', The Journal of Laboratory and Clinical Medicine, vol. 89, no. 2, pp. 250-256.
Higgins JR, Wambach G, Kem DC, Gomez-Sanchez C, Holland OB, Kaplan NM. Interaction of 16β-hydroxydehydroepiandrosterone with renal mineralocorticoid receptors. The Journal of Laboratory and Clinical Medicine. 1977;89(2):250-256.
Higgins, John R. ; Wambach, Gerhard ; Kem, David C. ; Gomez-Sanchez, Celso ; Holland, O. Bryan ; Kaplan, Norman M. / Interaction of 16β-hydroxydehydroepiandrosterone with renal mineralocorticoid receptors. In: The Journal of Laboratory and Clinical Medicine. 1977 ; Vol. 89, No. 2. pp. 250-256.
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