Interaction of packaging motor with the polymerase complex of dsRNA bacteriophage

Jiří Lísal, Denis E. Kainov, TuKiet T. Lam, Mark Emmett, Hui Wei, Paul Gottlieb, Alan G. Marshall, Roman Tuma

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Many viruses employ molecular motors to package their genomes into preformed empty capsids (procapsids). In dsRNA bacteriophages the packaging motor is a hexameric ATPase P4, which is an integral part of the multisubunit procapsid. Structural and biochemical studies revealed a plausible RNA-translocation mechanism for the isolated hexamer. However, little is known about the structure and regulation of the hexamer within the procapsid. Here we use hydrogen-deuterium exchange and mass spectrometry to delineate the interactions of the P4 hexamer with the bacteriophage phi12 procapsid. P4 associates with the procapsid via its C-terminal face. The interactions also stabilize subunit interfaces within the hexamer. The conformation of the virus-bound hexamer is more stable than the hexamer in solution, which is prone to spontaneous ring openings. We propose that the stabilization within the viral capsid increases the packaging processivity and confers selectivity during RNA loading.

Original languageEnglish (US)
Pages (from-to)73-79
Number of pages7
JournalVirology
Volume351
Issue number1
DOIs
StatePublished - Jul 20 2006
Externally publishedYes

Fingerprint

Capsid
Product Packaging
Bacteriophages
RNA
Viruses
Deuterium
Adenosine Triphosphatases
Hydrogen
Mass Spectrometry
Genome

Keywords

  • Assembly
  • Dynamics
  • Hydrogen-deuterium exchange
  • Interactions
  • Packaging
  • RNA
  • Structure

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Lísal, J., Kainov, D. E., Lam, T. T., Emmett, M., Wei, H., Gottlieb, P., ... Tuma, R. (2006). Interaction of packaging motor with the polymerase complex of dsRNA bacteriophage. Virology, 351(1), 73-79. https://doi.org/10.1016/j.virol.2006.03.025

Interaction of packaging motor with the polymerase complex of dsRNA bacteriophage. / Lísal, Jiří; Kainov, Denis E.; Lam, TuKiet T.; Emmett, Mark; Wei, Hui; Gottlieb, Paul; Marshall, Alan G.; Tuma, Roman.

In: Virology, Vol. 351, No. 1, 20.07.2006, p. 73-79.

Research output: Contribution to journalArticle

Lísal, J, Kainov, DE, Lam, TT, Emmett, M, Wei, H, Gottlieb, P, Marshall, AG & Tuma, R 2006, 'Interaction of packaging motor with the polymerase complex of dsRNA bacteriophage', Virology, vol. 351, no. 1, pp. 73-79. https://doi.org/10.1016/j.virol.2006.03.025
Lísal, Jiří ; Kainov, Denis E. ; Lam, TuKiet T. ; Emmett, Mark ; Wei, Hui ; Gottlieb, Paul ; Marshall, Alan G. ; Tuma, Roman. / Interaction of packaging motor with the polymerase complex of dsRNA bacteriophage. In: Virology. 2006 ; Vol. 351, No. 1. pp. 73-79.
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