Interaction with PCNA is essential for yeast DNA polymerase η function

Lajos Haracska, Christine M. Kondratick, Ildiko Unk, Satya Prakash, Louise Prakash

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162 Citations (Scopus)

Abstract

In both yeast and humans, DNA polymerase (Pol) η functions in error-free replication of ultraviolet-damaged DNA, and Polη promotes replication through many other DNA lesions as well. Here, we present evidence for the physical and functional interaction of yeast Polη with proliferating cell nuclear antigen (PCNA) and show that the interaction with PCNA is essential for the in vivo function of Polη. Polη is highly inefficient at inserting a nucleotide opposite an abasic site, but interaction with PCNA greatly stimulates its ability for nucleotide incorporation opposite this lesion. Thus, in addition to having a pivotal role in the targeting of Polη to the replication machinery stalled at DNA lesions, interaction with PCNA would promote the bypass of certain DNA lesions.

Original languageEnglish (US)
Pages (from-to)407-415
Number of pages9
JournalMolecular Cell
Volume8
Issue number2
DOIs
StatePublished - 2001

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Proliferating Cell Nuclear Antigen
DNA-Directed DNA Polymerase
Yeasts
DNA
Nucleotides

ASJC Scopus subject areas

  • Molecular Biology

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Interaction with PCNA is essential for yeast DNA polymerase η function. / Haracska, Lajos; Kondratick, Christine M.; Unk, Ildiko; Prakash, Satya; Prakash, Louise.

In: Molecular Cell, Vol. 8, No. 2, 2001, p. 407-415.

Research output: Contribution to journalArticle

Haracska, Lajos ; Kondratick, Christine M. ; Unk, Ildiko ; Prakash, Satya ; Prakash, Louise. / Interaction with PCNA is essential for yeast DNA polymerase η function. In: Molecular Cell. 2001 ; Vol. 8, No. 2. pp. 407-415.
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