Interactions among the SNARE proteins and complexin analyzed by a yeast four-hybrid assay

Alvin M. Shih, Ok Ho Shin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Exocytosis is one of the most crucial and ubiquitous processes in all of biology. This event is mediated by the formation of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes, ternary assemblies of syntaxin, SNAP23/SNAP25 (synaptosomal-associated protein of 23 or 25 kDa), and synaptobrevin. The exocytotic process can be further regulated by complexin, which interacts with the SNARE complex. Complexin is involved in a Ca2+-triggered exocytotic process. In eukaryotic cells, multiple isoforms of SNARE proteins are expressed and are involved in distinct types of exocytosis. To understand the underlying biochemical mechanism of various exocytotic processes mediated by different SNARE protein isoforms, we systematically analyzed the interactions among syntaxin, SNAP23/SNAP25, synaptobrevin, and complexin by employing a newly developed yeast four-hybrid interaction assay. The efficiency of SNARE complex formation and the specificity of complexin binding are regulated by the different SNARE protein isoforms. Therefore, various types of exocytosis, occurring on different time scales with different efficiencies, can be explained by the involved SNARE complexes composed of different combinations of SNARE protein isoforms.

Original languageEnglish (US)
Pages (from-to)107-111
Number of pages5
JournalAnalytical Biochemistry
Volume416
Issue number1
DOIs
StatePublished - Sep 1 2011

Fingerprint

SNARE Proteins
Yeast
Assays
Yeasts
Exocytosis
Protein Isoforms
R-SNARE Proteins
Qa-SNARE Proteins
Synaptosomal-Associated Protein 25
Eukaryotic Cells

Keywords

  • Complexin
  • Exocytosis
  • SNAP23
  • SNAP25
  • SNARE complex
  • Synaptobrevin
  • Syntaxin
  • Yeast four-hybrid interaction assay

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Interactions among the SNARE proteins and complexin analyzed by a yeast four-hybrid assay. / Shih, Alvin M.; Shin, Ok Ho.

In: Analytical Biochemistry, Vol. 416, No. 1, 01.09.2011, p. 107-111.

Research output: Contribution to journalArticle

Shih, Alvin M. ; Shin, Ok Ho. / Interactions among the SNARE proteins and complexin analyzed by a yeast four-hybrid assay. In: Analytical Biochemistry. 2011 ; Vol. 416, No. 1. pp. 107-111.
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